Representing a renewable source for cell replacement, neural stem cells have received substantial attention in recent years. The neurosphere assay represents a method to detect the presence of neural stem cells, however owing to a deficiency of specific and definitive markers to identify them, their quantification and the rate they expand is still indefinite. Here we propose a mathematical inte...

in this center, from 1991 to 2002, 89 chronic myelogenous leukemic (cml) patients, age ranging between 8-48 years with a median age of 29, underwent hematopoietic stem cell transplantation. eighty-eight patients were in the first chronic phase of disease. twenty-three patients received bone marrow transplantation (bmt) and 66 patients received peripheral blood stem cell transplantations (pbsct)...

purpose: to report the early results of transplantation of autologous limbal stem cell cultivated on amniotic membrane in total limbal stem cell deficiency. methods: four eye of 4 patients with total unilateral stem cell deficiency secondary to severe chemical or thermal burn were included. stem cell deficiency was confirmed with impression cytology in all cases. under topical anesthesia, a sma...

two basic and clinical researches accomplished during the recent years on embryonic and adult stem cells constituted a mutation in regenerative therapy. these cells can be used for treating some degenerative diseases. between them, age-related functional defects, hematopoietic and immune system disorders, heart failures, chronic liver injuries, diabetes, parkinson’s and alzheimer’s diseases, ar...

background: because of the ability of proliferation, regeneration, conversion to differentiated cells and tissue producing, stem cell therapy has important clinical implications for treatment of different diseases. in this chapter we discuss different kinds of stem cells that have been used in treatment of neurologic diseases, gastrointestinal diseases, heart diseases, bone disease, kidney dise...

objective(s): osteoarthritis is one of the most common diseases in middle-aged population in the world. cartilage tissue engineering (te) has been presented as an effort to introduce the best combination of cells, biomaterial scaffolds and stimulating growth factors to produce a cartilage tissue similar to the natural articular cartilage. in this study, the chondrogenic potential of adipose der...

introduction: doxorubicin is frequently used for treatment of several types of cancer. doxorubicin cardiac toxicity has limited the use of this drug. corticosteroids may prevent doxorubicin induced cardiotoxicity. therefore the aim of this study was to evaluate mouse embryonic stem cells derived cardiomyocytes as a model to evaluate the effect of doxorubicin and dexamethasone. methods: mouse em...

Introduction: Cardiac glycosides such as digoxin or digitoxin are the natural products that are traditionally used to increase cardiac contractile force in patients with heart failure and cardiac arrhythmias. It has been shown that digoxin can directly inhibit the cell proliferation and lead to cell apoptosis. Present study was conducted to analyze the effect of digoxin in the cohorts of ...

AIM To find the mechanisms by which special AT-rich sequence-binding protein 2 (SATB2) influences colorectal cancer (CRC) metastasis. METHODS Cell growth assay, colony-forming assay, cell adhesion assay and cell migration assay were used to evaluate the biological characteristics of CRC cells with gain or loss of SATB2. Sphere formation assay was used to detect the self-renewal ability of CRC...

BACKGROUND Recent evidence suggests that head and neck squamous cell carcinomas (HNSCCs) harbor a small subpopulation of highly tumorigenic cells, designated cancer stem cells. A limiting factor in cancer stem cell research is the intrinsic difficulty of expanding cells in an undifferentiated state in vitro. METHODS Here, we describe the development of the orosphere assay, a method for the st...