The zebrafish has emerged as a valuable genetic model system for the study of developmental biology and disease. Zebrafish share a high degree of genomic conservation, as well as similarities in cellular, molecular, and physiological processes, with other vertebrates including humans. During early ontogeny, zebrafish embryos are optically transparent, allowing researchers to visualize the dynam...

By cutting gill filaments in anesthetized rainbow trout ( Oncorhynchus mykiss), observing the bleeding through a stereomicroscope, and using blockers of various known endogenous filament artery vasoconstrictors, we have here attempted to characterize hemostatic mechanisms in gills. The immediate hemostatic response to a cut in a gill filament artery was a local vasoconstriction, stopping the he...

The effects of vermicomposts on plant parasitic, fungivorous and baterivorous nematode populations were investigated in grape (Vitis vinifera) and strawberry (Fragaria ananasa) field crops. Commercially-produced vermicomposts derived from recycled paper, and supermarket food waste were applied to replicated plots at the rates of 2.5 t ha–1 or 5.0 t ha–1 for the grape crop and 5.0 t ha–1 or10 t ...

A mutant strain, PBA322, was constructed by electroporation of a phagemid containing the coding region of antisense RNA of the ompH gene, encoding 39 kDa capsular protein or OmpH, into the parental strain P-1059 (serovar A:3) of Pasteurella multocida, and the pathogenicity was determined in mice and chickens. Grayish colonies of the mutant, indicating loss of capsule synthesis, were observed un...

The middle ear ossicles are important due to transmission of sound to the inner ear leading to sound understanding. The aim of present study was to determine the anatomical and morphometrical aspects of middle ear ossicles in Makouei sheep fetuses. For this experimental study, eight sheep fetuses at the age of 2 to 3 months were provided from public slaughterhouse; their middle ear ossicles wer...

Porous oligolactide-hydroxyapatite composite scaffolds were obtained by stereolithographic fabrication. Gentamicin was then coated on the scaffolds afterwards, to achieve antimicrobial delivery ability to treat bone infection. The scaffolds examined by stereomicroscope, SEM, and μCT-scan showed a well-ordered pore structure with uniform pore distribution and pore interconnectivity. The physical...

The Root ZX has been introduced recently as a device capable of performing accurately in the presence of sodium hypochlorite, blood, water, local anesthetic, and pulpal tissues. The Root ZX was used to locate the apical foramen in 26 root canals of vital teeth. After extraction of the teeth, a stereomicroscope was used to confirm visually the relationship of the tip of the endodontic file to th...

Clonogenic assay or colony formation assay is an in vitro cell survival assay based on the ability of a single cell to grow into a colony. The colony is defined to consist of at least 50 cells. The assay essentially tests every cell in the population for its ability to undergo ‘‘unlimited’’ division. Clonogenic assay is the method of choice to determine cell reproductive death after treatment w...

INTRODUCTION The aim of this innovative study was to regenerate a condition that makes it possible to carry out researches in the field on a variety of resorption. MATERIALS AND METHODS In order to develop apical resorption, the root canals of selected teeth were instrumented and then drilled with #4 Gates Glidden drills. In the next stage, the teeth were submerged in melted rose wax up to 3 ...

The pharynx is a neuromuscular pump at the anterior end of the alimentary tract. It is made up of 20 muscle cells, 20 neurons, and 20 other cells. Pharyngeal activity correlates with food intake. The proper feeding rate, as well as the precise timing of pharyngeal movements, is required for efficient feeding and likely for survival in nature. For most purposes, pharyngeal behavioral analysis re...