Stool specimens were examined for the parasite Trichuris trichiura from 1,860 patients in 17 hospitals for the mentally subnormal in the U.K. It was found in 10 of these hospitals, and an overall incidence of 13.2% was recorded. Most cases occurred in the South, where the overall incidence was 36.3%.Difetarsone was used in the treatment of infestations in two hospitals, and proved to be very we...

Performance in proficiency testing (PT) schemes is an objective measure of a laboratory's best performance. We examined the performance of participants in two parasitology PT schemes in South Africa from 2004 through 2010. The average rates of acceptable scores over the period were 58% and 66% for the stool and blood parasite schemes, respectively. In our setting, participation in PT alone is i...

Fasciola hepatica is a worldwide distributed zoonotic trematode incidentally infecting humans. Although often symptomatic, fascioliasis can cause a wide spectrum of disease. The diagnosis can be established by stool examination detecting ova of the parasite, although serological testing has a higher sensitivity and specificity in the acute phase of disease. This case presents a 24-year-old Soma...

Performance in proficiency testing (PT) schemes is an objective measure of a laboratory’s best performance. We examined the performance of participants in two parasitology PT schemes in South Africa from 2004 through 2010. The average rates of acceptable scores over the period were 58% and 66% for the stool and blood parasite schemes, respectively. In our setting, participation in PT alone is i...

Stool samples for parasitological examination were collected in a remote area of western Nepal. Of 40 specimens collected, 36 were positive for parasites as determined by examination of direct wet mounts and trichrome smears. All but one of the positive specimens contained several parasite species, averaging four species per specimen. Four negative specimens were found in infants under 1 year o...

Samples were collected and stored at −80°C for ≥1 week to avoid the risk of infection with any Echinococcus spp. eggs present. Approximately 250 mg was placed into 2-mL tubes, heated at 65°C for 15 min, and stored at −80°C. The heating and cooling procedure helps to break the parasite egg shells, enabling more efficient DNA extraction. DNA was extracted by using the QIAamp DNA Stool Mini Kit (Q...

Dientamoeba fragilis is a protozoan parasite of humans that infects the mucosa of the large intestine and is associated with gastrointestinal disease. We developed a 5' nuclease (TaqMan)-based real-time PCR assay, targeting the small subunit rRNA gene, for the detection of D. fragilis in human stool specimens and compared its sensitivity and specificity to conventional PCR and microscopic exami...

OBJECTIVE To compare the frequency and etiology of diarrhea in children aged less than 2 years with known HIV status. METHODS This was a nested cohort study, whereby children were followed during monthly routine and unscheduled visits. The HIV status of children was determined with PCR. A stool culture was obtained from children with diarrhea. A subset of stool samples was examined for parasi...

Introduction: Polyparasitism remains one of the most pressing public health problems with third world population generally estimated to be infected. Also, Women are particularly concern due their gender roles which is sensitive since iil and/or illness family members represent greater burdens for poor women in comparison men.
 Aim: To epidemiologically study polyparasitism Reproductive age...