39 Applying in silico simulations and in vitro experiments, the amino acid proline was proved to 40 have a profound influence on Streptomyces griseus trypsinogen, and the hydrogen bond 41 between H and D was crucial for trypsin activity. By introducing an artificial propeptide 42 IVEF, the titer of trypsin was increased by 6.71-fold. 43

An earlier paper (Lechevalier, Acker, Corke, Haenseler, and Waksman, 1953), announcing the addition of candicidin to a rapidly growing list of antifungal antibiotics, described the morphological and cultural characteristics of Streptomyces griseus 3570, from which candicidin originated, its selective antibiotic action, and methods by which the agent itself could be isolated from culture broths ...

There is considerable evidence for the existence of alternate pathways of glucose dissimilation in microbial systems (1). Several organisms belonging to the genus Xtreptomyces have been investigated by some workers (2, 3). Hockenhull et al. (4), Ganguly and Roy (5), and Wang et al. (6) have particularly studied Streptomyces griseus and showed that this organism utilizes both the glycolytic and ...

The streptomycin-producing capacity of submerged cultures of Streptomyces griseus 107 was nearly lost during repeated transfers on synthetic nutrient medium. This effect did not occur on the same nutrient medium from which Fe(++) was omitted. The physiological reason for this effect was discussed from the point of the relationship of iron-containing porphyrin enzymes to the process of streptomy...

New antibiotics, griseusins A and B were isolated from a strain of Streptomyces griseus. Both antibiotics have a 5-hydroxy-1, 4-naphthoquinone chromophore and their molecular formulae were determined to be C22H20O10 and C22H22O10, respectively. The griseusins are active against gram-positive bacteria.

Within recent years, phages capable of infecting various Streptomyces species have been reported (Saudek and Colingsworth, 1947; Reilly et al., 1947; Koerber et al., 1950; Perlman et al., 1951; and Alexander and McCoy, 1956). Hoehn (1949) and subsequently Gilmour and Buthala (1950) demonstrated that streptomyces phage is indigenous to soil, the natural habitat of the host. The streptomyces host...

The nucleotide sequence of the Streptomyces griseus afsA gene, possibly encoding a key enzyme for A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) biosynthesis, was determined. The translational initiation codon was identified by introducing out-of-frame mutations at appropriate positions by oligonucleotide-directed mutagenesis. The afsA gene was thus found to code for a protein o...

The antimicrobial activities of twenty-two substances structurally related to staurosporine, aglycone in the indolocarbazole and bis-indole series were examined against Streptomyces chartreusis and Streptomyces griseus, Bacillus cereus, Escherichia coli, Candida albicans and Botrytis cinerea. Inhibition of sporulation was examined also on the two species of Streptomyces. Unlike literature repor...

Streptomycetes are mycelial bacteria that produce sporulating aerial hyphae on solid media. Bald (bld) mutants fail to form aerial mycelium under at least some conditions. bldA encodes the only tRNA species able to read the leucine codon UUA efficiently, implying the involvement of a TTA-containing gene in initiating aerial growth. One candidate for such a gene was bldH, because the bldH109 mut...