نتایج جستجو برای: stx2
تعداد نتایج: 1191 فیلتر نتایج به سال:
The 2011 outbreak of infections with Escherichia coli with characteristics of both enteroaggregative E. coli (EAEC) and Shiga toxin-producing E. coli (STEC) caused a paradigm shift with regard to the human pathogenicity of STEC (1). Diarrheagenic E. coli (DEC) usually does not cause extraintestinal diseases such as urinary tract infections and bacteremia (2). The DNA of 193 E. coli isolates fro...
Today, it is nearly 25 years past from investigation of Shiga toxigenic Escherichia coli (STEC) which is able to produce Shiga toxins and cause different gastroenteritis. Since incidence of gastroenteritis due to STEC is increasing, it's necessary to develop rapid, specific and accurate procedures like PCR. In this study, we used PCR method to detect and identify STEC in cultures of 55 Escher...
Abstract The consumption of raw milk cheese has been growing worldwide with S. aureus and E. coli the leading agents in food poisoning. present work aims to evaluate microbiological quality cheeses commercialized Brazil, regarding microbiology safety enterotoxin gene presence. Forty-three samples from five different suppliers were analyzed. Counting identification aureus, Salmonella spp perform...
Abstract The current study included the investigation of E. coli bacteria in random samples ground meat, detection virulence genes isolates, and isolates resistance to some widely used antibiotics. Twenty-four minced meat were collected isolate identify by phenotypic, microscopic characteristics traditional biochemical tests done. Antibiotic susceptibility tests, genomes extracted, factors Stx1...
background: increase of people’s awareness about side effects of chemical food preservatives has raised public interest to consume products with natural preservatives such as essential oils. garlic (allium sativum l.) is one of the medicinal plants in traditional medicine of iran and it is necessary to evaluate its antimicrobial effects on some food borne bacteria such as e.coli o157:h7. this b...
Shiga toxin-producing Escherichia coli have been associated with food-borne illnesses. Pasteurization is used to inhibit microbial growth in milk, and an open question is whether milk pasteurization inactivates Shiga toxins. To answer this question we measured Shiga toxin's inhibition effect on Vero cell dehydrogenase activity and protein synthesis. Our data demonstrate that Shiga toxin 2 (Stx2...
OBJECTIVE Tissue plasminogen activator (tPA), a serine protease, catalyzes the conversion of plasminogen to plasmin, the major enzyme responsible for endogenous fibrinolysis. In some populations, elevated plasma levels of tPA have been associated with myocardial infarction and other cardiovascular diseases. We conducted a meta-analysis of genome-wide association studies to identify novel correl...
A method has been developed to identify the α-subunit of Shiga toxin 2 (α-Stx2) from Escherichia coli O157:H7 using matrix-assisted laser desorption/ionization time-of-flight-time-of-flight tandem mass spectrometry (MALDI-TOF-TOF-MS/MS) and top-down proteomics using web-based software developed in-house. Expression of Stx2 was induced by culturing E. coli O157:H7 on solid agar supplemented with...
The macrophage has previously been implicated in contributing to the renal inflammation associated with hemolytic-uremic syndrome (HUS). However, there is currently no in vivo model detailing the contribution of the renal macrophage to the kidney disease associated with HUS. Therefore, renal macrophage recruitment and inhibition of infiltrating renal macrophages were evaluated in an established...
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