Accumulation of environmentally hazardous agricultural waste, in particular cow manure without the proper treatment, creates an ecological threat to environment. During energy crisis, search for alternative pathways obtain is also one most urgent issues today. Cow promising as a substrate anaerobic fermentation with production valuable carrier — methane. Fermentation sole not spread. It takes p...

The three branched-chain amino acids (BCAAs) are the most hydrophobic of the amino acids and play crucial roles in determining the structures of globular proteins as well as the interaction of the transmembrane domains of membranous proteins with phospholipid bilayers. However, the three BCAAs do not behave identically. In terms of protein secondary structure, valine and isoleucine exhibit a de...

Insulin receptor substrates (IRS) serve as downstreammessengers from activated cell surface receptors to numerous signaling pathway cascades. One of these pathways, phosphoinositide 3-kinase (PI3K), frequently displays aberrant function in the setting of cancer. IRS proteins are capable of both regulating and activating PI3K, depending on the cell of origin. As such, both prohost and protumor f...

Barrett, T., Maryanka, D., Hamlyn, P. M. & Gould, H. J. (1974). Proc. Nutl. Acud. Sci. U.S.A. 71,5057-5061 Chiu, J. F., Tsai, Y. H., Sakuma, K. & Hnilica, L. S. (1975) J. Biol. Chem. 250,9431-9433 Dickson, R. C., Abelson, J., Barnes, W. M. & Reznikoff, W. S. (1975) Science 187,27-35 Gilmour, R. S. & MacGillivray, A. J. (1976) Proc. Meet. SOC. Dev. Biol., in the press Gilmour, R. S. & Paul, J. (...

DesB, which is derived from Sphingobium sp. SYK-6, is a type II extradiol dioxygenase that catalyzes a ring opening reaction of gallate. While typical extradiol dioxygenases show broad substrate specificity, DesB has strict substrate specificity for gallate. The substrate specificity of DesB seems to be required for the efficient growth of S. sp. SYK-6 using lignin-derived aromatic compounds. S...

Four unresolved issues of cyclin-dependent kinase (CDK) regulation have been addressed by structural studies this year - the mechanism of CDK inhibition by members of the INK4 family of CDK inhibitors, consensus substrate sequence recognition by CDKs, the role of the cyclin subunit in substrate recognition and the structural mechanism underlying CDK inhibition by phosphorylation.

Based on the hypothesis of Pavlov [1] and early experiments with rats [2, 3], it was thought that diets rich in either fats, proteins or carbohydrates would cause a selective increase of synthesis of those pancreatic enzymes corresponding to the predominant substrates. Although correct when diets contained at least 15 percent protein, this concept had to be revised, because in extreme situation...

The activation segment of protein kinases is structurally highly conserved and central to regulation of kinase activation. Here we report an atypical activation segment architecture in human MPSK1 comprising a beta sheet and a large alpha-helical insertion. Sequence comparisons suggested that similar activation segments exist in all members of the MPSK1 family and in MAST kinases. The consequen...

Under substrate adherent conditions, integrin gene expression can be regulated by transforming growth factor-beta, interleukin-1 beta, and prostaglandins. This report demonstrates a new mechanism that can differentially control the expression of several integrins. When MG-63 osteosarcoma cells are maintained in suspension, up-regulation of several integrin alpha-subunits takes place. Within as ...

The effects of diabetes on myocardial metabolism are complex in that they are tied to the systemic metabolic abnormalities of the disease (hyperglycemia and elevated levels of free fatty acid and ketone bodies), and changes in cardiomyocyte phenotype (e.g., down-regulation of glucose transporters and PDH activity). The cardiac adaptations appear to be driven by the severity of the systemic abno...