Reactive oxygen and nitrogen species such as superoxide and nitric oxide are released into the extracellular spaces by inflammatory and airway epithelial cells. These molecules may exacerbate lung injury after influenza virus pneumonia. We hypothesized that enhanced expression of extracellular superoxide dismutase (EC SOD) in mouse airways would attenuate the pathological effects of influenza p...

PURPOSE Pancreatic ductal adenocarcinoma (PDA) cells are known to produce excessive amounts of reactive oxygen species (ROS), particularly superoxide, which may contribute to the aggressive and refractory nature of this disease. Extracellular superoxide dismutase (EcSOD) is an antioxidant enzyme that catalyzes the dismutation of superoxide in the extracellular environment. This study tests the ...

Recent studies have demonstrated an induction of expression of inducible nitric oxide synthase that is associated with several inflammatory diseases of the skin. To define the mechanisms of action of nitric oxide (NO) in the skin, we attempted to identify genes that are regulated by NO in keratinocytes. Using the human keratinocyte cell line HaCaT as a model system, we identified a Cu/Zn supero...

Aging is an universal, intrinsic, progressive, irreversible and deleterious phenomenon. As age advances, several enzymes show an increase, while some decrease and some do not show any change in their activities. These specific alterations in the enzymes must have inflicted a great impact on the process of aging. This process is thought to be related to increase in free radicals generation and o...

Recently, a novel electron spin resonance (ESR) spin-trapping agent, 2-(diphenylphosphinoyl)-2-methyl-3,4-dihydro-2H-pyrrole N-oxide (Diphenyl-PMPO), was synthesized. Because it had some advantages in stability and reactivity over conventional spin-trapping agents, we applied it to the ESR analyses of superoxide anion radical scavenging activity (SOSA) of superoxide dismutase (SOD) and of well-...

In airway diseases, smooth muscle cells can proliferate at exaggerated rates; thus, the identification of endogenous pathways that limit proliferative responses is important. Here we show that human airway smooth muscle express type I nitric oxide synthase (NOS), which results in inhibition of DNA synthesis and cell proliferation. In addition, superoxide dismutase (SOD), a cell-permeable mimeti...

trichomoniasis, the most widespread sexually transmitted disease is caused by trichomonas vaginalis. this parasite is site specific for the genitourinary tract and recruitment of macrophages as well as polymorphonuclear nutrophils (pmn) to the site of infection is the first line of defense as a component of non-specific resistance and immunity. in this study, balb/c mice were infected with 10 i...

The mitochondrial matrix is a metabolically differentiated intracellular space concerning superoxide (O2 -), nitric oxide (NO) and peroxynitrite (ONOO) metabolism because of the impermeability of the mitochondrial inner membrane to O2 -, H+ and ONOOand the specific presence of relatively high concentrations of Mn-superoxide dismutase (Mn-SOD), about 3-10 μM.1 Superoxide anion (O2 -) is formed t...

This study was performed to investigate the transduction of a full-length superoxide dismutase (SOD) protein fused to transactivator of transcription (Tat) into human chondrocytes, and to determine the regulatory function of transduced Tat-SOD in the inflammatory cytokine induced catabolic pathway. The pTat-SOD expression vector was constructed to express the basic domain of HIV-1 Tat as a fusi...

background: non-steroidal anti-inflammatory drugs (nsaid) have been associated with antioxidant property and have been shown to improve the circulating antioxidant status on daily dosing in different inflammatory conditions. the present study was conducted to investigate the antioxidant role of meloxicam in aluminum induced oxidative stress in rat brain. methods: in the in vivo experiments, spr...