conjugation of monoclonal antibodies to super paramagnetic nanoparticles is an effective method for cancer diagnosis and treatment. in this study the humanized anti her2/neu monoclonal antibody- herceptin- was conjugated to super paramagnetic iron oxide (spio) nanoparticles using edc method. the concentration of the conjugated antibodies was measured by bradford assay. the antibody-nanoparticle...

Since metals are cable of catalysing the free radical reactions, different metal (Mg, Mn, Zn) compounds were studied to determine their hydroxyl radical generating and/or scavenging abilities depending on ligand in a H2O2/OH -luminol system with or without microperoxidase. Magnesium oxide, magnesium gluconate and manganese gluconate increase the free radical reactions in the H2O2/OH -luminol sy...

Introduction: Iron plays an important role in physiological processes as a trace element. Today, iron oxide nanoparticles have attracted extensive attention due to their super paramagnetic properties and a variety of potential applications in many fields. The main objective of this study was to evaluate in vitro and in vivo toxic effects of the iron oxide nanoparticles on L929 cell line, kidney...

Bauhinia variegata L. is traditionally used in treating a variety of ailments in India. Phytochemical screening and in vitro free radical scavenging activity of aqueous and ethanolic bark extracts of Bauhinia variegata was assessed by studying its ability to scavenge DPPH, Nitric oxide, hydroxyl radical and reducing power. Phytochemical analysis revealed the presence of steroid, phenol/ tannin,...

Anion exchange membranes (AEM) based on polyphenylene oxide (PPO) suffered quaternary-ammonium-cation-site degradation in alkaline environments. Surprisingly, the degradation rate was considerably faster in the presence of molecular oxygen. We postulated that the AEM cation-site catalyzes the reduction of dioxygen by hydroxide ions to yield the superoxide anion radical and the highly reactive h...

The aim of this study was to investigate the antioxidant activity using different in vitro assays and nitric oxide synthase activation property of crude water soluble polysaccharide from P. ostreatus. The polysaccharide was isolated by hot water extraction and physico-chemical investigation revealed that it contained high amount of carbohydrate (mostly β-glucan) and low amount of protein and ph...

The human body has only a limited toolbox to prevent and control acute and chronic diseases. A important cell type involved in our immune protection is the monocyte/macrophage which possesses a major inducible chemistry linked to the excessive possible production of gaseous free radical nitric oxide (.NO) produced via nitric oxide synthase 2 (inducible NOS2). In addition, these activated cells ...

Andrographis paniculata has been used as a traditional medicine and a predominant constituent in 26 Ayurvedic formulations over the last two decades. Its impact on the male fertility is a controversial from the past few years. In the present study we have investigated the antioxidant enzyme status and the indices of lipid peroxidation in the testis and epididymis of male wistar rats. Male wista...

Background & Aims: The activity of nitric oxide synthase was assayed in enterocytes isolated from human duodenal biopsies to determine its role in celiac disease. Patients were categorized into those with irritable bowel syndrome, iron deficiency anemia, B12/folate deficiency and treated and untreated celiac disease. Methods & Results: Enterocytes isolated from all groups showed 1400W-inhibitab...