Plackett-Burman design was used to efficiently select important medium components affecting the lipase production by Aspergillus niger using shea butter cake as the main substrate. Out of the eleven medium components screened, six comprising of sucrose, (NH4)2SO4, Na2HPO4, MgSO4, Tween-80, and olive oil were found to contribute positively to the overall lipase production with a maximum producti...

The immobilization of Rhizopus oryzae lipase (RoL) by hydrophobic adsorption on polypropylene supports with additives was investigated. Additives such as hen egg albumin, sodium caseinate and CAVAMAX® W6 were used to coat the support during where immobilized RoL coated compared those noncoated support. Following immobilization, catalytic activity characterized based different temperatures pH. w...

A recombinant Serratia sp. W3 lipase (rSmL) was expressed in E. coli and purified to homogeneity. The kinetic properties, regio-selectivity, interfacial performances of rSmL were compared with those the native (nSmL). has a molecular mass 67 kDa specific activity 3530 U mg−1 which is around 12-fold higher than that nSmL (300 mg−1) when using olive oil as substrate. Both able hydrolyze ester bon...

A superhydrophobic surface is coated on the surface of a cloth by MTCS (methyltrichlorosilane) nanoparticles and then, it is modified by SiO2–m–FTCS (1H,1H,2H,2H-Perfluorododecyltrichlorosilane) nanoparticles. This surface is characterized by SEM(scanning electron microscopy), and FT-IR (Fourier transform-infrared microscopy) and also its contact angle is investigated in various conditions and ...

Hydroxyapatite coatings have been used on metallic substrates in a variety of applications, including modifying the surface of human implants, bone osseointegration and biological fixation. In this paper, the effects of various kinds of metallic substrate on clinical and pathological results of in vivo tests are presented. Four kinds of endodontic implants i.e, stainless steel, cobalt base all...

Hydroxyapatite coatings have been used on metallic substrates in a variety of applications, including modifying the surface of human implants, bone osseointegration and biological fixation. In this paper, the effects of various kinds of metallic substrate on clinical and pathological results of in vivo tests are presented. Four kinds of endodontic implants i.e, stainless steel, cobalt base all...

A series of poly(GMA-co-EGDMA) resins with identical composition but varying particle sizes, pore radii, specific surface areas and specific volumes are studied to assess how Candida antarctica lipase B immobilization affects the porosity of the copolymer particles. Mercury porosimetry reveals a significant change in the average pore size (up to 6.1-fold), the specific surface area (up to 3.2-f...

Directed evolution strategy (error-prone PCR) was conducted to improve the activity of lipase from Rhizopus chinensis CCTCC M201021. Through two rounds of ep-PCR and pNPP top agar screening, two optimum mutant strains 1-11 and 2-28 were obtained with 2 and 4 fold of enzyme activity higher than that of parent strain, respectively. DNA sequencing of mutant lipase 2-28 revealed four amino acid sub...

The requisite initial reaction for in vivo thrombus formation in flowing blood is platelet adhesion to the exposed surface of the extracellular matrix. The contribution of von Willebrand factor (vWF ) in plasma and glycoprotein (GP) Ib on the platelet membrane to platelet adhesion has been well-documented. We have recently developed a procedure (the "flow adhesion assay") for measuring platelet...

Magnetic silica aerogel in hydrophobic and hydrophilic forms were used as support to immobilize Candida rugosa lipase by adsorption method. Response surface methodology (RSM) was employed to study the effects of the three most important immobilization parameters, namely enzyme/support ratio (0.3-0.5, w/w), immobilization time (60-120 min) and alcohol percentage (20-40, %v/v) on the specific act...