Valeriana officinalis (valerian), as a nutraceutical herb, is widely used for its sedative and hypnotic properties. It is known that C15 sesquiterpenoid valerenic acid (VA) is the active ingredient responsible for pharmacological effects of V. officinalis. To evaluate the effect of methyl jasmonate (MeJA) concentrations (50 and 100 µM...

The ATP synthase in mitochondria was responsible for the synthesis of to provide chemical energy cell achieve metabolism. point mutation at mitochondrial DNA nt 8993(T>C and T>G) disrupted normal cellular mechanism synthase, causing a deficiency production synthesis. 8993 causes replacement leucine amino acid with an arginine(aL156R), changing sequence ATP6 gene, which inefficiency c-subu...

The complete mRNA sequence of one tobacco (nicotiana tabacum) gene—guanosine monophosphate (GMP)synthase, was amplified using the rapid amplification cDNA ends methods. full-length GMP synthase gene 2,127bp containing a 1,617 bp open reading frame, which encodes protein 538 amino acids. Sequence analysis revealed that shares high homology with wood tobacco(99%), nicotiana attenuata(99%), toment...

The identification of genes involved in the adaptive evolution of non-model organisms with uncharacterized genomes constitutes a major challenge. This study employed a rigorous and targeted candidate gene approach to test for positive selection on protein-coding genes of the blue crab, Callinectes sapidus. Four genes with putative roles in physiological adaptation to environmental stress were c...

A systematic screen of volatile terpene production in the glandular trichomes of 79 accessions of Solanum habrochaites was conducted and revealed the presence of 21 mono- and sesquiterpenes that exhibit a range of qualitative and quantitative variation. Hierarchical clustering identified distinct terpene phenotypic modules with shared patterns of terpene accumulation across accessions. Several ...

Abstract Terpene synthases (TPS) are responsible for the terminal biosynthetic step of terpenoid production. They encoded by a highly diverse gene family believed to evolve tandem duplication in response adaptive pressures. Taxa Myrtaceae renowned their diversity terpenoid-rich essential oils, and among them, tribe Eucalypteae has largest TPS found any plant (> 100 ). In this study, comparat...

Genes for trehalose metabolism are widespread in higher plants. Insight into the physiological role of the trehalose pathway outside of resurrection plant species is lacking. To address this lack of insight, we express Escherichia coli genes for trehalose metabolism in Arabidopsis thaliana, which manipulates trehalose 6-phosphate (T6P) contents in the transgenic plants. Plants expressing otsA [...

Terpenes constitute the largest class of plant secondary (or specialized) metabolites, which are compounds of ecological function in plant defense or the attraction of beneficial organisms. Using biochemical and genetic approaches, nearly all Arabidopsis thaliana (Arabidopsis) enzymes of the core biosynthetic pathways producing the 5-carbon building blocks of terpenes have been characterized an...

1, 4, 5, 6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid (ectoine) is an excellent osmoprotectant. ectoine and hydroxyl ectoines are of great significance to the biotechnology industry, thus the detection and isolation of ectoine producing bacteria is of great importance. hence, this study involved the detection of the ectc gene (encoding ectoin synthase enzyme) using polymerase chain reacti...

Valeriana officinalis (valerian), as a nutraceutical herb, is widely used for its sedative and hypnotic properties. It is known that C15 sesquiterpenoid valerenic acid (VA) is active ingredient responsible for pharmacological effects of V. officinalis. To evaluate the effect of methyl jasmonate (MeJA) concentrations (50 and 100 µM) in the modulation of expression patterns of the genes involved ...