The worldwide commercial production of the oyster mushroom Pleurotus ostreatus is currently threatened by massive attacks of green mold disease. Using an integrated approach to species recognition comprising analyses of morphological and physiological characters and application of the genealogical concordance of multiple phylogenetic markers (internal transcribed spacer 1 [ITS1] and ITS2 sequen...

The present research deals with the effect of postharvest treatment of grape berries with four commonly used fungicides and two forms of Trichoderma harzianum on the infection with soft-rot fungiRhizopus stolonifer and Mucor piriformis. This effect was evaluated by comparison of the external diameter of rot-lesion in treated and untreated berries, in addition to comparison of percent reduction ...

Media and culturing protocols were identified that supported the formation of submerged conidia and microsclerotia (MS) by Trichoderma harzianum Rifai strain T-22 using liquid culture fermentation. Liquid media with a higher carbon concentration (36 g L(-1)) promoted MS formation at all C:N ratios tested. Hyphae aggregated to form MS after 2 d growth and after 7 d MS were fully melanized. This ...

Different species of Trichoderma especially T. harzianum (Th2 biotype in Europe and Th4 in America) considered as causal agent of green mould disease on button mushroom (Agaricus bisporus) casing beds. In this study, 100 fungal isolates were collected and purified from different mushroom growing units in Tehran province and Urmia (NW Iran). Totally, 60 isolates of T. harzianum, 27 isolates of T...

Verticillium wilt is a serious disease of pistachio caused by Verticilliumdahliae. Control of the disease is difficult due to soil borne nature of the causal agent. Verticillium wilt has been biologically controlled by Trichoderma spp. In the present study, Trichoderma spp. was isolated from soils of pistachio orchards and their effect was investigated on radial growth of Verticillium dahliae b...

Two biological control agents, Bacillus subtilis AP-01 (Larminar(™)) and Trichoderma harzianum AP-001 (Trisan(™)) alone or/in combination were investigated in controlling three tobacco diseases, including bacterial wilt (Ralstonia solanacearum), damping-off (Pythium aphanidermatum), and frogeye leaf spot (Cercospora nicotiana). Tests were performed in greenhouse by soil sterilization prior to i...

Several Trichoderma spp. are well known for their ability to: (i) act as important biocontrol agents against phytopathogenic fungi; (ii) function as biofertilizers; (iii) increase the tolerance of plants to biotic and abiotic stresses; and (iv) induce plant defense responses via the production and secretion of elicitor molecules. In this study, we analyzed the gene-regulation effects of Trichod...

BACKGROUND Basal stem rot (BSR) disease caused by the fungus Ganoderma boninense is the most serious disease affecting the oil palm; this is because the disease escapes the early disease detection. The biocontrol agent Trichoderma harzianum can protect the disease only at the early stage of the disease. In the present study, the expression levels of three oil palm (Elaeis guineensis Jacq.) chit...

The presence of phorbol esters (PEs) with toxic properties limits the use of Jatropha curcas kernel in the animal feed industry. Therefore, suitable methods to detoxify PEs have to be developed to render the material safe as a feed ingredient. In the present study, the biological treatment of the extracted PEs-rich fraction with non-pathogenic fungi (Trichoderma harzianum JQ350879.1, T. harzian...

A new semi-selective medium, acidified weak potato-dextrose agar (AWPDA) with Mertect (active ingredient: thiabendazole), was developed for the isolation and enumeration of Alternaria species from samples of soil and plant debris. The medium was selected based on growth inhibition tests against Alternaria and several other commonly encountered saprobic fungi utilizing three antifungal agents, B...