To evaluate cell mediated immunopathogenic mechanisms in chronic hepatitis B virus (HBV) infection, we investigated the changes of T4/T8 ratios from the peripheral blood, the percentages of IL-2 receptor expression after stimulation of mitogens (Con A, PHA) and a specific antigen (Hepatitis type B surface antigen, HBs), and the proliferative response mediated by IL-2 receptors after rIL-2 stimu...

Topotecan- or mitoxantrone-selected cell lines (T8 and MX3, respectively), derived from the human IGROV1 ovarian cancer cell line, were resistant to the topoisomerase I inhibitors topotecan, SN-38 (the active metabolite of irinotecan), and 9-aminocamptothecin, as well as to the topoisomerase II drug mitoxantrone. In both resistant cell lines, decreased accumulation of topotecan and mitoxantrone...

Purpose: Enteroaggregative Escherichia coli (EAEC) is an emerging foodborne pathogen as a significant diarrheal pathogen in multiple population groups. However, the associated phenotypic changes by which EAEC bacteria survive acid conditions, as it passes through the human digestive track, is not completely understood. Materials and methods: EAEC (T8) was grown in vitro by mimicking the in vivo...

A reverse haemolytic plaque forming cell (PFC) assay was employed to analyse the impact of T suppressor/cytotoxic and T helper cells on B cell function in 10 patients with rheumatoid arthritis (RA). In all cases T8-enriched cells from synovial fluid and blood suppressed the pokeweed mitogen (PWM) induced IgM, IgG, and IgA secretion by autologous lymphocytes to the same degree. The suppression w...

We determined the effect of an H1 receptor antagonist on the functional recovery of Carassius auratus submitted to telencephalic ablation. Five days after surgery the fish underwent a spatial-choice learning paradigm test. The fish, weighing 6-12 g, were divided into four groups: telencephalic ablation (A) or sham lesion (S) and saline (SAL) or chlorpheniramine (CPA, ip, 16 mg/kg). For eight co...

Pseudomonas aeruginosa is the predominant pathogen associated with chronic lung infection among cystic fibrosis patients. During colonization of the lung, P. aeruginosa converts to a mucoid phenotype characterized by the overproduction of the exopolysaccharide alginate. Secretion of newly synthesized alginate across the outer membrane is believed to occur through the outer membrane protein AlgE...

This investigation uses different polyclonal activators of in vitro immunoglobulin production to elicit immunoregulatory profiles of B cells, T cells, T4 cells, and T8 cells from 25 recipients (13 with and 12 without chronic graft-v-host disease [GVHD] ) after HLA-identical marrow transplantation for aplastic anemia or hematologic malignancy. Pokeweed mitogen, Epstein-Barr virus, herpes simplex...

To cite: Iliescu L, Toma L. BMJ Case Rep Published online: [please include Day Month Year] doi:10.1136/ bcr-2013-201070 DESCRIPTION A 59-year-old patient presented with a 2-month history of increasing backache irradiating in the abdomen. All standard laboratory tests results were within the normal limits, with the exception of a moderate inflammatory syndrome. Lower limb paralysis occurred soon...

Treatment of human breast epithelial cells MCF-10F with 17-β-estradiol has been reported to result in E2-transformed cells which have given rise to highly invasive C5 cells that in turn generate tumors in SCID mice. From these tumors, various cell lines, among which C5-A6-T6 and C5-A8-T8, were obtained. Although different phases of the tumorigenesis process in this model have been studied in mo...

Two monocyte-derived cytokines, interleukin-1 (IL-1) and interleukin-6 (IL-6), have been reported to costimulate monocyte-depleted T cell populations in the presence of mitogen, and this effect has been attributed to an accessory function of these molecules. We have now examined further the accessory function potential of IL-1 plus IL-6, and examined how these cytokines promote T cell growth wi...