Plectranthus amboinicus (Lour.) Spreng is a perennial plant from the mint family with aromatic, succulent leaves and several health benefits. Multiple shoot regeneration was accomplished in vitro using nodal segments (NS) explants of P. pretreated 0, 0.5, 5, 25, 50, 100 μM thidiazuron (TDZ) for 4 h, then transferred to growth regulator-free media. After 8 weeks growth, NS pre-treated 25 TDZ h T...

the present study was carried out to investigate micropropagation possibility and determine the optimal medium composition and plant growth regulators (pgrs) combinations under in vitro conditions. the cotyledon and epicotyl explants obtained from 1 month old in vitro grown seedlings were used in ms medium containing ba (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) and tdz (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) e...

A method for tissue culture of micropropagation aquatic plant Staurogyne repens (Nees) Kuntze has been developed. Different phytohormones were investigated to optimize the growth explants. The shoot tip explants S. placed in MS nutrient medium including BAP (0.30-1.50 mg/l) + TDZ (0.10 NAA and Kn mg/l). Shoot regeneration frequencies changed as 77.77-100% 66.66-100% cultivation augmented with B...

Background: A consistent practice for mass multiplication of banana cv. Malnad Rasbale has been accomplished, which is an endemic cultivar Western Ghats Karnataka, India. Methods: Decrease in microbial contagion have managed by dealing with outward sterilization agents ethanol, sodium hypochlorite, mercuric chloride solution, antibiotic solutions containing cefotaxime and gentamicin. MS media a...

The Dendrobium variety orchid is a plant that has rare potential and growth. This requires large amounts of seeds can be propagated in vitro. study aims to determine the effect TDZ on direct induction somatic embryogenesis. was conducted using completely randomized design (CRD) with one factor, namely media consisting basal Murashige Skoog (MS) growth regulator TDZ. Each treatment out total sev...

Considering great medicinal value of Hyssopus officinalis L. and possibility of its mass production through in vitro culture, two individual experiments was conducted. Effect of various concentrations (0, 2.2, 4.4 and 11 µmol) of TDZ and BAP in combination with 1 µmol of IAA on direct regeneration from nodal explants were assessed. Significant difference between treatments was observed (P≤ 0.01...

The present study was carried out to investigate micropropagation possibility and determine the optimal medium composition and plant growth regulators (PGRs) combinations under in vitro conditions. The cotyledon and epicotyl explants obtained from 1 month old in vitro grown seedlings were used in MS medium containing BA (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) and TDZ (0.1, 0.2, 0.5, 1, 2 and 3 mg-1) e...