Foreign secretory pathway proteins are often produced in surprisingly low amounts in the baculovirus/insect cell expression system. One possible reason for this is that heterologous signal peptides might be inefficiently recognized by the insect cell protein translocation machinery. This idea was supported by a recent study showing that secretion of a plant protein in the baculovirus system was...

Seasonal variations in pore water and solid phase geochemistry were investigated in urbanized minerotrophic peat sediments located in southwestern Michigan, USA. Sediment pore waters were collected anaerobically, using pore water equilibrators with dialysis membranes (“peepers”) and analyzed for pH, alkalinity, dissolved PO−3 4 , NH + 4 , S −2, SO−2 4 , Fe +3, Fe+2, and Mn+2 at 1–2 cm intervals...

Unstructured models for cell growth, xylose consumption and xylitol production were applied to describe the fermentation kinetics of using Candida tropicalis in synthetic medium,and non-detoxified oil palm empty fruit bunch (OPEFB) hydrolysate at 100 mL flask scale. In medium, experimental maximum specific growth rate (μmax) mass yield factor(YX/S) closer results Tessier model than those Contoi...