Homogeneous biosynthetic sn-glycerol-3-phosphate dehydrogenase (EC 1.1.1.8) of Escherichia coli was potently inhibited by palmitoyl-CoA and other long chain acyl-CoA thioesters. The concentration dependence of this inhibition was not cooperative. Enzyme activity was inhibited 50% at 1 microM palmitoyl-CoA; thus, this inhibition occurred at concentrations below the critical micellar concentratio...

Two enzymes were purified from actinomycin-synthesizing Streptomyces chrysomallus which could be identified as peptide synthetases involved in the biosynthesis of actinomycin. Actinomycin synthetase II activates the first two amino acids of the peptide chains of the peptide lactone antibiotic, threonine and valine (or isoleucine), as thioesters via their corresponding adenylates. It is a single...

Galloyl-Coenzyme A, 4-0-/?-D-Glucosidogalloyl-Coenzyme A, Thioester, 4-0-/?-D-Glucosidogalloyl Hydroxamic Acid, N-Succinimidyl 4-0-/?-D-Glucosidogallate, Gallotannins Galloyl-CoA, a potential intermediate in the biosynthesis of gallotannins, has been prepared via N-succinimidyl 4-0-/?-D-glucosidogallate and 4-0-/?-D-glucosidogalloyl-CoA. Besides a major absorption band at 261 nm, the UV-spectra...

/3-Hydroxyacyl acyl carrier protein (ACP) dehydrase has been purified 2900-fold from extracts of Escherichia coli. The enzyme catalyzes the reversible dehydration of /3-hydroxyacyl-ACP thioesters to yield specifically tram-2-enoylACP products. It is active with frans-2-enoyl-ACP thioesters of chain lengths from 4 through 16 carbon atoms. The enzyme catalyzes the hydration of cis-5frans-2-dodeca...

Experimental data on the stereoselectivity of base-catalyzed 1,2-elimination reactions that produce conjugated carbonyl compounds are scarce in spite of the importance of these reactions in organic and biochemistry. As part of a comprehensive study in this area, we have synthesized stereospecifically-deuterated beta-tosyloxybutanoate esters and thioesters and studied the stereoselectivity of th...

Di(4-nitrobenzoyloxy)iodo]benzene (1), a hypervalent iodine compound, is easy to prepare and stable as solid, was shown be useful functional group transforming agent in this report. Oxidative synthesis of symmetrical disulfides from thiols the presence compound 1 alone performed 58–100% yields. Furthermore, ESI-MS demonstrated that premixing Ph3P yields corresponding acyloxyphosphonium, which c...