in this paper, a new topology is proposed for three-phase to single-phase matrix converters, where more voltage levels are produced in the output in comparison with the conventional topologies. in addition, a new control method based on minimum error between the generated and the desired output voltages is proposed for three-phase to single-phase matrix converters. in the proposed control metho...

Three phase partitioning (protein precipitate obtained as an interfacial layer between lower aqueous and upper t-butanol phases, formed by the addition of ammonium sulphate and t-butanol to the aqueous solution of protein) followed by lyophilization in the presence of two-component excipient resulted in 400-480x increases in transesterification activity of lyophilized powders of subtilisin Carl...

Precipitation of alpha chymotrypsin in the simultaneous presence of ammonium sulphate and t-butanol (three phase partitioning) resulted in preparations which showed self aggregation of the enzyme molecules. Precipitation with increasing amounts of ammonium sulphate led to increasing size of aggregates. While light scattering estimated the hydrodynamic diameter of these aggregates in the range o...

A strategy called macro-(affinity ligand) facilitated three-phase partitioning (MLFTPP) is described for refolding of a diverse set of recombinant proteins starting from the solubilized inclusion bodies. It essentially consists of: (i) binding of the protein with a suitable smart polymer and (ii) precipitating the polymer-protein complex as an interfacial layer by mixing in a suitable amount of...

Separation of Trametes versicolor laccase from a commercial preparation was carried out using Three-Phase-Partitioning (TPP) technique. The conditions were optimized and the most favorable ammonium sulphate concentration (70% w/v), crude to t-butanol ratio (1.0:2.0) and pH (4.5) resulted in 56.22% yield with 1.65-fold purity of laccase. For further improvement in purification, an improved versi...

Starch-degrading enzymes glucoamylase (from Aspergillus niger), and pullulanase (from Bacillus acidopullulyticus) were purified using alginates (polysaccharides consisting of mannuronic acids and guluronic acids) by a recently developed technique called macroaffinity ligand-facilitated three-phase partitioning (MLFTPP). In this process, a crude preparation of the enzyme was mixed with alginate....

An understanding of the gas/particle-phase partitioning of semivolatile compounds is critical in determining atmospheric aerosol formation processes and growth rates, which in turn affect global climate and human health. The Study of Organic Aerosol at Riverside 2005 campaign was performed to gain a better understanding of the factors responsible for aerosol formation and growth in Riverside, C...

Grouping is a global partitioning process that integrates local cues distributed over the entire image. We identify four types of pairwise relationships, attraction and repulsion, each of which can be symmetric or asymmetric. We represent these relationships with two directed graphs. We generalize the normalized cuts criteria to partitioning on directed graphs. Our formulation results in Raylei...