Mice immunized with a glucan-killed parasite vaccine exhibited enhanced resistance to Leishmania donovani infection as evidenced by decreased hepatic parasite burdens when compared to unvaccinated controls. This resistance was not seen in mice immunized with killed parasites alone. Glucan vaccination resulted in increased resistance at day 6, but this effect was no longer present by day 20 of t...

s of the Fifth International Complement Workshop, Hotel Del Coronado, Coronado, California, February 23-25. 1973 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 NUMBZR 2, AUGUST 1973

The upstream activation site (UAS) of the yeast phosphoglycerate kinase gene (PGK) has been localised by deletion analysis (1). Here we show that the UASPGK contains two functionally distinct domains. These two domains, designated activator (A) and modulator (M), appear to be located within bases -460 to -402 and -531 to -461, respectively, relative to the initiating ATG; although it is possibl...

The International Symposium on Auto­ phagy (ISA) is an important meeting with a long history devoted solely to the topic of autophagy. Yoshimori Ohsumi (Tokyo Institute of Technology, Japan), who identified a series of autophagy­related genes (ATG genes) in yeast [1], proposed and organized the first ISA in Okazaki, Japan, in 1997. Over the years, the ISA sym­ posia have witnessed the explosive...

Large doses of antihuman lymphoblast globulin (AHLG) were used prophylactically in three recipients of renal allografts from living related donors. These recipients received 1, 500 mg/day of AHLG for 14 days begining on the second day before transplantation in addition to the standard immunosuppression. Serum AHLG levels, serum anti-AHLG antibodies and T cell subsets were checked serially. The ...

Here, we present a universal, simple, efficient, and reliable way to add small BioBrick parts to any BioBrick via PCR that is compatible with BioBrick assembly standard 10. As a proof of principle, we have designed a universal primer, rbs_B0034, that contains a ribosomal binding site (RBS; BBa_B0034) and that can be used in PCR to amplify any coding BioBrick that starts with ATG. We performed t...

The vast majority of studies addressing the induction of autophagy have focused upon cytoplasmic aspects of its regulation. Recently, we have started to expand our knowledge regarding the nuclear events of autophagic induction. Many autophagy-related genes are transcriptionally upregulated upon induction of autophagy, but only in a limited number of cases do we know the pathways leading to this...