3D bioprinting additively assembles bio-inks to manufacture tissue-mimicking biological constructs, but with the typical building blocks limited 1D filaments. Here, it is developed a voxelated technique for digital assembly of spherical particles (DASP), which are effectively 0D voxels—the basic unit structures. It shown that DASP enables on-demand generation, deposition, and viscoelastic bio-i...

Stem/progenitor cell based therapeutic approach in our daily routine clinical practice, has been elusive dream in medical sciences and improvement of stem cell homing as one of major challenges in cell therapy programs, has been considered a promising milestone. It has been proved that stem/progenitor cells exhibit a homing response to injured tissues/organs, mediated by interactions of chemoki...

Tissue engineered constructs have the potential to respond unmet medical need of treating deep osteochondral defects. However, current tissue engineering strategies struggle in attempt create patterned with biologically distinct functionality. In this work, a developmentally-inspired modular approach is proposed, whereby cartilaginous organoids are used as living building blocks. First, hierarc...

Native extracellular matrix (ECM) is a complex fibrous structure loaded with bioactive cues that affects the surrounding cells. A promising strategy to mimicking native tissue architecture for tissue engineering applications is to engineer fibrous scaffolds using electrospinning. By loading appropriate bioactive cues within these fibrous scaffolds, various cellular functions such as cell adhesi...

Breast reconstruction is a type of surgery for women who have had a mastectomy, and involves using autologous tissue or prosthetic material to construct a natural-looking breast. Adipose tissue is the major contributor to the volume of the breast, whereas epithelial cells comprise the functional unit of the mammary gland. Adipose-derived stem cells (ASCs) can differentiate into both adipocytes ...

Rebuilding damaged or diseased tissue by means of biological additive manufacturing has recently gained lot attention and shown very promising result. Using biofabrication techniques to mimic replicate natural as well cell environment is a capable way achieve physiologically relevant conditions. Especially in electrophysiological human like cardiac neural tissue, proper signal transduction para...

Tissue engineered heart muscle may be able to provide a treatment modality for early stage congestive heart failure. In this study, we describe a new method to engineer functional 3-dimensional heart muscle utilizing a biodegradable fibrin gel. Primary cardiac myocytes were isolated from hearts of 2- to 3-day-old rats and processed in one of the two ways. For the first method (layering approach...

Engineered tissues need a vascular network to supply cells with nutrients and oxygen after implantation. A network that can connect to the vasculature of the patient after implantation can be included during in vitro culture. For optimal integration, this network needs to be highly organized, including venules, capillaries, and arterioles, to supply all of the cells with sufficient nutrients. O...

The materials pipeline for biomaterials and tissue engineering applications is under continuous development. Specifically, there is great interest in the use of designed materials in the stem cell arena as materials can be used to manipulate the cells providing control of behavior. This is important as the ability to "engineer" complexity and subsequent in vitro growth of tissues and organs is ...