To determine the prevalence of goblet cell metaplasia in endocervical and endometrial adenocarcinomas by histochemial staining and to investigate the most sensitive histochemical staining method to detect this metaplasia, a total of 90 tissue blocks representing 30 non-neoplastic cervix, 30 non-neoplastic endometrium, 30 endocervical and endometrial adenocarcinoma cases were obtained for histoc...

Photoactivated disinfection has a strong local antimicrobial effect. In the field of dentistry it is an emerging adjunct to mechanical debridement during endodontic and periodontal treatment. In the present study, we investigate the effect of photoactivated disinfection using riboflavin as a photosensitizer and blue LED light for activation, and compare it to photoactivated disinfection with th...

Testicular damage is one of the most hazardous effects as it’s associated with azoospermia. Busulfan (Bu) a highly toxic chemotherapeutic drug that affects testis. Thirty male Swiss albino mice divided into six groups 5 animals each. Control (oral 0.9% saline daily for 75 days); Mel (20 mg/kg/day orally 30 ZnO NPs (5 i.p. BU (single injection 40 mg/kg and then left 45 + dose days followed by 20...

background: cell-based treatment approach using differentiated mesenchymal stem cells (mscs) and mature chondrocytes has been considered as an advanced treatment for cartilage repair. we investigated the differentiated level of these two cell types that is crucial for their repair capacity for cartilage defect at a co-culture micro mass system.   methods: passaged-2 mscs isolated from the mouse...

Background:-Mitotic figures refer to the various phases of mitosis which are well appreciated in tissue sections.They act as prognostic indicators in oral epithelial dysplasia. Aim:-To compare and evaluate the mitotic figures in oral epithelial dysplasia using H & E and Toluidine blue stains. Objective:-Using H&E, Toluidine blue stains in the identification and counting of mitotic figures in di...

Early detection of oral premalignant lesions (OPMLs) by visual inspection with toluidine blue has not been addressed. We conducted a community-based randomized controlled trial to assess whether using toluidine blue as an adjunctive tool for visual screening had a higher detection rate of OPMLs and could further reduce the incidence of oral cancer. In 2000, in Keelung, we randomly assigned a to...

OBJECTIVE To study the effects of velvet antler polypeptides (VAPs) on osteoarthritic chondrocytes (OCs) in rabbits. METHODS An osteoarthritic rabbit model was established according to Hulth's method. OCs were isolated and cultured for observation of the cell cycle. Cell proliferation was detected by MTT assay and the cell cycle was monitored by flow cytometry. The phenotype was determined by...

The diagnosis and treatment of oral premalignant lesions and squamous cell carcinoma are currently based on histopathologic features, site of involvement and stage of disease. Recent advances in techniques for detecting lesions and predicting their progression or recurrence are reviewed here. Adjuncts for detection of lesions and selection of biopsy sites include vital tissue staining (with tol...

A simple procedure for selective staining of acidic nuclear proteins is described, in which toluidine blue 0 was employed at pH 9. The acidic proteins are present in nucleoli, the nuclear ribonucleoprotein network, the region of nuclear membrane, and the cytoplasm. They are found also in intimate association with chromosomes in mitotic figures, and form a thin peripheral layer around them. Hist...