High quality small RNA extraction is a critical aspect in miRNA expression profiling. Various RNA isolation methods using commercially available kits can extract total RNA enriched for small RNA. However, each of these protocols yields small RNA preparations that can differ in total quantity and quality, which may affect downstream experiments. This Application Note demonstrates the use of the ...

Background: RNA interference (RNAi) is a phenomenon uses double-stranded RNA (dsRNA) to specifically inhibit gene expression. The non-specific silencing caused by interferon response to dsRNA in mammalian cells limits the potential of utilizing RNAi to study gene function. Duplexes of 21-nucleotide short interfering dsRNA (siRNA) inhibit gene expression by RNAi. In some organisms, siRNA can als...