Plants respond to pest attack, among other mechanisms, by producing specific proteins with insecticidal properties. Proteins toxic effects on insects have also been discovered in many organisms, especially fungi and bacteria. Due their biological function, represent an important potential the development of more environmentally friendly plant protection methods. Increasing knowledge about mode ...

Protein misfolding is the molecular basis for several human diseases. How the primary amino acid sequence triggers misfolding and determines the benign or toxic character of the misfolded protein remains largely obscure. Among proteins that misfold, polyglutamine (polyQ) expansion proteins provide an interesting case: Each causes a distinct neurodegenerative disease that selectively affects dif...

Bacillus thuringiensis EG2838 and EG4961 are highly toxic to Colorado potato beetle larvae, and only strain EG4961 is toxic to southern corn rootworm larvae. To investigate the cause of the different insecticidal activities of EG2838 and EG4961, cryIII-type genes toxic to coleopterans were cloned from each strain. The cryIIIB gene, cloned as part of an 8.0-kb EcoRI fragment of EG2838 DNA, encod...

The cowpea weevil (Callosobruchus maculatus) is the main pest that attacks (Vigna unguiculata) seeds during storage, causing nutritional and economic losses in crop. Thus, studies aiming to identify resistant cultivars have been developed. Chitin-binding proteins (CBP), such vicilins chitinases, detected related with toxicity insects. In this work, we investigated presence of chitin-binding par...

Although originally thought to be an inhibitor of PDK-1, OSU-03012 (AR-12) was shown in 2005 not to primarily act as a PDK-1 inhibitor in its ability to radio-sensitize tumor cells [1,2]. Subsequently it was shown that the primary mechanism by which AR-12 killed tumor cells was via the PKR-like endoplasmic reticulum kinase (PERK) -dependent induction of endoplasmic reticulum stress signaling [1...

The aim of this work is to present the experimental results in form an algorithm for analyzing modification screen printed electrodes, including possibility its regeneration irreversibly oxidizing biologically active compounds (drugs, DNA and proteins). A protocol was developed quantitative analysis study mechanism drug-DNA interaction by differential pulse voltammetry, following parameters: co...

Background: Apis cerana honey bee is a native to Asia that spreads from Afghanistan, China, and Japan Indonesia. The productivity of bees can produce as much 2-5 kg per colony in year. Royal jelly the queen's nourishment, it produced by larvae, adult bees, young worker male larvae. CBAVD contributes 1–2% 20–25% males with subfertility. Aim: objective this study was evaluate Jelly Cerana potenti...

Genomic DNA extraction with a high quantity and quality is a basic requirement in molecular biology. The DNA obtained was free of any contamination proteins, polysaccharide, polyphenols and colored pigments. These compounds would interfere with the genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The isolated genomic DNA was fo...

Surfaces coated with polyzwitterions are known to resist protein adhesion and be generally bio-inert. In recent reports, several polyzwitterionic coatings carboxylate groups intrinsic antimicrobial activity due the pH-responsivity of that group described, but design rules obtain such remain unclear. Therefore, in this work, a set surface-attached varying alkyl residues is studied. The gradually...

Abstract Background The E. coli pET system is the most widely used protein over-expression worldwide. It relies on assumption that all cells produce target and it generally believed integral membrane (IMP) more toxic than their soluble counterparts. Results Using GFP-tagged proteins, high level of either or IMP targets results in > 99.9% cell loss with survival rate only < 0.03%. Selectiv...