The objective of this manuscript was to assess the toxicity formation on biological treated wastewater from a municipal wastewater treatment plant, after a chlorination-dechlorination disinfection process. Water was monitored by means of physicochemical and ecotoxicological parameters, with the use of Vibrio fischeri bioassay. Since a dechlorination process removed residual chlorine, the positi...

Fall armyworm, Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae), is a destructive pest of wide array agricultural and horticultural crops worldwide. This in vitro research assessed the combined effect methanolic extracts indigenous flora Soone Valley (Khushab, Pakistan) nine commonly used synthetic insecticides against 3rd instar larvae S. using leaf-dip bioassay method. Toxicity bioas...

An enzyme-linked immunosorbent assay (ELISA) for Clostridium botulinum type A and type B toxins was assessed for diagnostic accuracy in cases of infant botulism. This test was positive in all 22 cases confirmed by the conventional tests, which included the mouse lethality assay and stool culture. Stool specimens from five cases were positive by culture, but the mouse lethality bioassay was eith...

the acute toxicity of a malaysian crude oil to red snapper, lutjanus argentimaculatus, blacktiger shrimp and penaeus monodon were investigated. the animals were exposed to water solublefractions (wsf) of crude oil in a flow-through bioassay system and the toxicants were analyzed by gaschromatography (gc) and gas chromatography and mass spectrometer (gc-ms). the 96 h lc50 valuesof the wsf of cru...

The human monoclonal antibody CP-870,893 is a CD40 receptor agonist currently being developed for the treatment of cancer. A bioassay to measure neutralizing antibodies (Nab) to CP-870,893 in 5% human serum matrix was developed and validated utilizing the Daudi cell line and flow cytometric detection. Additionally, samples from CP-870,893 treated cynomolgus monkeys were analyzed in the bioassay...

In this study, we performed ecotoxicity tests of sodium arsenite on soil invertebrate Eisenia fetida. Initially, broad range of sodium arsenite (0.1, 1, 10, 100mg/ml) was tested for mortality of E. fetida after 24 hours in filter paper test. The narrow range doses (0-1mg/ml) were further tested to determine the EC50 and LD50. The EC50 dose of sodium arsenite was considered at which 50% of earth...