We generated a CD8 T-cell receptor (TCR) transnuclear (TN) mouse specific to the Ld -restricted immunodominant epitope of GRA6 from Toxoplasma gondii as a source of cells to facilitate further investigation into the CD8 T-cell-mediated response against this pathogen. The TN T cells bound Ld -Gra6 tetramer and proliferated upon unspecific and peptide-specific stimulation. The TCR beta sequence o...

Toxoplasma immunoglobulin E (IgE) antibodies were evaluated in an immunosorbent agglutination assay (ISAGA) and an enzyme-linked immunosorbent assay (ELISA) to determine their usefulness in the diagnosis of acute infection with Toxoplasma gondii. IgE antibodies were not detected in serum specimens from otherwise seronegative individuals, individuals with chronic toxoplasma infection, or infants...

Introduction and objective: Toxoplasma gondii is an intra cellular protozoan parasite which infects 30 to 60% of the world population. A wide range of toxoplasmosis conditions has been studied, but there are still unknown damages which must be explored. In the present research, the effect of this parasite on testosterone and cortisol changes in the infected men and women was examined. Materials...

congenital toxoplasmosis is associated with variable complications including encephalitis, microcephaly, hydrocephaly, hepatitis, lymphadenopathy and even intrauterine death. presence of toxoplasma gondii in human placenta may induce congenital infection. the aim of this study was to determine the genotypes of toxoplasma gondii infection in human spontaneous aborted fetuses in shiraz, south of ...

We determined the prevalence of Toxoplasma antibodies in 576 primigravid women randomly selected from health houses in Hamadan. The relationship between positivity and some risk factors was assessed. Data were collected by questionnaire and blood samples examined for Toxoplasma antibodies by indirect fluorescent antibody test. Of the 576 women, 193 (33.5%) were positive for Toxoplasma antibodie...

Cardiac biopsy samples taken from transplant recipients around the time of primary toxoplasma infection were investigated by conventional histology and amplification of the P30 gene of Toxoplasma gondii by the polymerase chain reaction (PCR). Toxoplasma was detected more frequently by PCR than histology which may reflect the enhanced sensitivity of the former technique. Further studies are requ...

Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission to the fetus. This study was conducted to test the utility of a polymerase chain reaction (PCR) assay to detect recent infections with Toxoplasma in pregnant women. One hundred forty-eight women with high-risk pregnancies who had abnormal pregnancy outcomes (cases) and 100 wi...

A 32-year-old man presented with agitation, headache, and confusion. He was immunocompetent and had been living with multiple cats for many years. His vital signs were stable. He was afebrile. Multiple blood tests did not show any serious problem. Brain magnetic resonance imaging (MRI) revealed multiple ring-enhancing white matter lesions. Cerebrospinal fluid analysis did not show any signs of ...

Acute Toxoplasma gondii infection in early pregnancy carries the risk of transmitting the infection to the fetus with serious sequelae. However, serological testing for IgG/IgM anti-Toxoplasma antibodies may fail to differentiate between a recent and past infection. Two hundred and twenty-four Kuwaiti women in their first trimester were screened for IgG/IgM antibodies by the Vitek Immuno Diagno...

BACKGROUND The aim of this study was to determine the Toxoplasma antibodies in pregnant women in Zanjan, by ELISA method. METHODS Blood samples were taken from 500 pregnant women referred to the health centers of Zanjan City, North West Iran, IgM and IgG titers were primarily evaluated. The collected data were analyzed with SPSS 11.5 using Chi-Square test. RESULTS Anti Toxoplasma IgM and Ig...