It has been reported that serum transaminase activity is found to be altered in certain pathological conditions which are associated with necrosis or other type of cellular damage of cardiac, hepatic or skeletal muscle tissues (5, 6 and 12). It may be that damage to the tissue results in release, from the cells, of the traasaminase, which finds its way into the plasma or serum. Wroblewski and L...

Enzymatic transamination consists of the enzyme catalyzed reversible transfer of the alpha amino nitrogen of an amino acid to an alpha-keto acid with the synthesis of a second amino acid and a second alpha-keto acid. Enzymes catalyzing different transamination reactions are found widely distributed in animal tissues and have been shown to change in activity in some tissues during disease (1-3)....

W/E RECENTLY reported a new colorimetric assay for glutamic-oxalacetic transaminase which involves coupling the oxalacetate formed in the enzymatic reaction with a diazonium salt (1, 2). Because of the greater specificity of the diazonium salt for the product of the reaction and the minimum number of manipulations involved, this procedure is simpler and more accurate than previously reported pr...

BACKGROUND A minority of persons at risk develop liver cirrhosis, but knowledge of risk modulators is sparse. Several reports suggest that coffee drinking is associated with lower cirrhosis risk. METHODS We studied 125,580 multiethnic members of a comprehensive prepaid health care plan without known liver disease who supplied baseline data at voluntary health examinations from 1978 to 1985. S...

‘THE RECENT DEVELOPMENT of a new colorimetric procedure (TransAc*), for the determination of serum glutamic oxalacetic transaminase utilizing the diazonium salt, 6-benzamido-4-methoxy-m-toluidine diazonium chloride, as the color developer with a specificity for oxalacetic acid, prompted the following experiments. Determination of glutamic oxalacetic transaminase activities were performed accord...

3. The presence of equimolar concentrations of pyridoxal phosphate prevented the inhibition by isoniazid. Stable hydrazones of isoniazid did not inhibit transaminase activity. These compounds, which were stable in the presence of heavy suspensions of B.C.G. organisms, had tuberculostatic activity comparable with isoniazid. 4. Transaminase activity was found in extracts of cells which had been e...

A novel beta-transaminase gene was cloned from Mesorhizobium sp. strain LUK. By using N-terminal sequence and an internal protein sequence, a digoxigenin-labeled probe was made for nonradioactive hybridization, and a 2.5-kb gene fragment was obtained by colony hybridization of a cosmid library. Through Southern blotting and sequence analysis of the selected cosmid clone, the structural gene of ...