Semecarpus anacardium L. is a tree species which produces secondary metabolites of medicinal importance. Roots of the plant have been traditionally used in folk medicines. Different strains of Agrobacterium rhizogenes (A4, ATCC15834 and LBA 9402) were used for induction of hairy roots in in vitro grown tissues of the plant. Hairy root initiation was observed after 25-30 days of infection. Optim...

This study was performed to investigate the effect of Ag+ as an elicitor on the activity of antioxidant enzymes, content of H2O2, accumulation of total tocopherol and antioxidant activity in hairy root cultures of Silybum marianum (L.) Gaertn. The hairy roots were treated with 2 mM Ag+. The treated and non-treated hairy roots were harvested in the same time and data on H2O2 content, DPPH, tocop...

The common soil bacteria Agrobacterium tumefaciens and Agrobacterium rhizogenes are unique genetic pathogens capable of fundamentally redirecting plant metabolism in order to generate macroscopic tissue masses (crown galls and hairy roots, respectively) which support the growth of large populations of Agrobacteria. Central to pathogenesis is the horizontal transfer of a suite of oncogenes from ...

background: elicitors can trigger defense responses and activate specific genes involved in secondary metabolite biosynthesis. objective: for understanding sa-treatment signaling pathway we determined defense-related compounds and enzymes in s. marianum hairy root cultures. methods: 28 day old hairy roots were treated by salicylic acid (6 mg/50 ml culture) for different times (24, 48, 72, 96 an...

Hairy roots of horseradish (Armoracia rusticana) were induced by inoculation with Agrobacterium rhizogenes harboring Ri plasmid and cultured on phytohormone-free Murashige and Skoog medium after eliminating the bacteria. Hairy roots grew vigorously and sometimes formed yellowish calli under dark conditions. On the other hand, growth of hairy roots stopped after several weeks of culture with lig...

This study evaluated the induction of hairy roots in Perovskia abrotanoides Karel, a medicinally important member of Lamiaceae family, using different strains of Agrobacterium rhizogenes and various carbon supplies, for the first time. This research established an efficient Agrobacterium mediated transformation system for this plant species through bacterial strains including ATCC15834, TR105 a...