Presented is an evaluation of phase contrast techniques in transmission electron microscopy. The traditional defocus phase contrast is compared to two recently developed phase plate techniques. One is the Zernike phase contrast transmission electron microscope, the other is the Hilbert differential contrast thransmission electron microscope. The imaging characteristics of each technique are dis...

We describe in detail, the design, construction, and testing of a specimen holder that allows for the nanoindentation of surfaces while viewing in cross-section in a high voltage transmission electron microscope (TEM). This nanoindentation specimen holder, having three-axis position control of a diamond indenter in combination with micromachined specimens, allows for the first time the dynamic ...

nano-sized γ-al2o3 was synthesized successfully by a simple method using al2(so4)3·18h2o as aluminium source and in the presence of polyethylene glycol (peg 2000). ftir spectroscopy, x-ray diffraction (xrd), scanning electron microscope (sem) and transmission electron microscope (tem) were used to characterize the structure and morphology of synthesized powder. the results showed that peg playe...

Nanocrystalline metals demonstrate a range of fascinating properties, including high levels of mechanical strength. However, as these materials are exposed to high temperatures, it is critical to determine the grain size evolution, as this process can drastically change the mechanical properties. In this work, nanocrystalline sputtered Cu thin films with 43 +/- 2 nm grain size were produced by ...

We demonstrate that accurate values of mass-per-length (MPL), which serve as strong constraints on molecular structure, can be determined for amyloid fibrils by quantification of intensities in dark-field electron microscope images obtained in the tilted-beam mode of a transmission electron microscope. MPL values for fibrils formed by residues 218-289 of the HET-s fungal prion protein, for 2-fo...

Background: Curcuma longa generally known as turmeric includes curcuminoids and sesquiterpenoids as components, which are known to have antioxidative, anticarcinogenic, and anti-inflammatory activities. Iron, magnetite, and hematite as a micronutrient play an important role in physiological and chemical processes. Chitosan is a natural polymer derived from chitin and is recognized as versatile ...

The combination of self-assembly (bottom up) and nano-imprint lithography (top down) is an efficient and effective way to record information at the nanoscale by writing. The use of an electron beam for writing is quite a promising strategy; however, the 'paper' on which to save the information is not yet fully realized. Herein, graphene was selected as the thinnest paper for recording informati...

Three-dimensional reconstruction of nano-scale objects (such as biological macromolecules) can be accomplished using data recorded with a transmission electron microscope. An image obtained by a transmission electron microscope can be conceived of as an ‘ideal’ projection subjected to a contrast transfer function, which attenuates most frequencies, reverses the phase of others and even eliminat...