BACKGROUND Despite being the most important cellulase producer, the cellulase-regulating carbon source signal transduction processes in Trichoderma reesei are largely unknown. Elucidating these processes is the key for unveiling how external carbon sources regulate cellulase formation, and ultimately for the improvement of cellulase production and biofuel production from lignocellulose. RESUL...

In this study, cellulase, endoglucanase, and ?-glucosidase activity produced by three fungal strains (Aureobasidium pullulans, Neurospora crassa Trichoderma reesei) were determined on differently processed canola substrates [hexane extracted meal (HECM), washed HECM (WHECM), seed sprout (CSM)] in mono- co-culture fermentation under solid- submerged- state processes. Results showed that the inoc...

BACKGROUND Bioethanol isolated from lignocellulosic biomass represents one of the most promising renewable and carbon neutral alternative liquid fuel sources. Enzymatic saccharification using cellulase has proven to be a useful method in the production of bioethanol. The filamentous fungi Acremonium cellulolyticus and Trichoderma reesei are known to be potential cellulase producers. In this stu...

BACKGROUND Trichoderma reesei is the main producer of lignocellulolytic enzymes that are required for plant biomass hydrolysis in the biorefinery industry. Although the molecular toolbox for T. reesei is already well developed, repressible promoters for strain engineering and functional genomics studies are still lacking. One such promoter that is widely employed for yeasts is that of the L-met...

Some 800 soil and rotten wood samples were collected from Gilan, Mazandaran and GolestanProvinces in the north of Iran. By using selective and general media, 217 Trichoderma isolates were obtained belonging to sections Longibrachiatum (69 isolates) and Pachybasium (148 isolates), respectively. Pure cultures were obtained by single conidium and single chain methods. Colony features were recorded...

Three fungal species were evaluated for their abilities to saccharify pure cellulose. The three species chosen represented three major wood-rot molds; brown rot (Gloeophyllum trabeum), white rot (Phanerochaete chrysosporium) and soft rot (Trichoderma reesei). After solid state fermentation of the fungi on the filter paper for four days, the saccharified cellulose was then fermented to ethanol b...

In order to efficiently utilize natural cellulose materials to produce ethylene, three expression vectors containing the ethylene-forming enzyme (efe) gene from Pseudomonas syringae pv. glycinea were constructed. The target gene was respectively controlled by different promoters: cbh I promoter from Trichoderma reesei cellobiohydrolases I gene, gpd promoter from Aspergillus nidulans glyceraldeh...

Dolichol phosphate mannose (DPM) synthase activity, which is required in N:-glycosylation, O-mannosylation, and glycosylphosphatidylinositol membrane anchoring of protein, has been postulated to regulate the Trichoderma reesei secretory pathway. We have cloned a T.reesei cDNA that encodes a 243 amino acid protein whose amino acid sequence shows 67% and 65% identity, respectively, to the Schizos...

The calcium and phosphorous retention of layers was evaluated at different production levels (45 75 95 % of final production peak) with Hy-Line Brown hybrid layers during the first twelve weeks of the laying cycle. 32 layers evenly distributed over 4 treatments (Trts). The Ca content of the diets was identical across all Trts (32,5 g/kg), while P contents and phytase activities differed. In the...

Hydrophobins are a group of small amphiphilic proteins which are known to self-assemble on interfaces. They contain eight conserved cysteine residues, which make four disulfide bridges. A new hydrophobin protein, HFBIII, from the fungus Trichoderma reesei contains one extra cysteine residue, giving the protein a naturally reactive site. The self-assembly of hydrophobin protein HFBIII was studie...