A method for preparing highly purified glutaminase (EC 3.5.1.2, L-glutamine amidohydrolase) from pig renal cortex is described. The main steps consist of sodium sulfate fractionation followed by alternative solubilization by dialysis against Tris-HCI buffer and precipitation with phosphateborate. The linal enzyme preparation contains no impurities which could be detected by sucrose gradient cen...

Various methods of reconstructing transcriptional regulatory networks infer transcriptional regulatory interactions (TRIs) between strongly coexpressed gene pairs (as determined from microarray experiments measuring mRNA levels). Alternatively, however, the coexpression of two genes might imply that they are coregulated by one or more transcription factors (TFs), and do not necessarily share a ...

An overview is given of the chemistry of tris(azolyl)phosphines with focus on their preparation and application in coordinationand organometallic chemistry and catalysis. These systems share with the more abundant tris(pyrazolyl)borates and -methanes the ability to function as tridentate nitrogen ligands with hemilabile character, but the additional phosphine donor site grants them bifunctional...

The 5'-nucleotidase of Escherichia coli was shown to be located at the cell wall surface by histochemical techniques utilizing the deposition of inorganic phosphate. Penetration of the 5'-nucleotidase in the periplasmic space was seen only in cells treated with ethylenediaminetetraacetic acid (EDTA)-tris(hydroxymethyl)aminomethane (Tris). The 3'-nucleotidase of E. coli was also found to have a ...

From Figure A, protein bands on SurePAGE were more evenly distributed compared to competitor B’s Tris-Glycine gel, in which proteins in the middle parts of the gel were clustered. In addition, more bands were visualized on SurePAGE compared to both competitor B and homemade Tris-Glycine gels (zoomed panel). Lastly, SurePAGE offers slightly better resolution compared to Competitor T’s Bis-Tris g...

The reaction of TiCl3 ⋅ 3THF with perfluoropinacol (H2fpin), triethylamine and silver nitrite in acetonitrile yielded the formation first tris(perfluoropinacolato) complex a transition metal, (HNEt3)3[TiIV(fpin)3]NO2 (1). compound was fully characterized IR UV-vis spectroscopy as well elemental analysis single crystal X-ray diffraction. structure 1 is compared that known homoleptic titanium(IV)...

BACKGROUND α-Bromination of the side chain of aromatic ketones using NBS in the presence of p-toluenesulfonic acid (p-TsOH) in acetonitrile is very common. However, regioselective bromination of bis and tris(ω-bromoacetophenones) with NBS in the presence of p-TsOH in acetonitrile under microwave irradiation is quite novel. The bis- and tris(ω-bromoacetophenones) are used in synthesis of bis and...