In order to facilitate optimal plant DNA quantitation and identification, an assay has been developed that uses generic plant PCR primers that amplify a region in the chloroplast genome of plant samples. The assay uses the SYBR green detection dye to detect the PCR product with a universal PCR primer set to the large subunit of ribulose bisphosphate carboxylase, rbcL, but can be used with any o...

Plants and their associated insect herbivores, represent more than 50% of all known species on earth. The first step in understanding the mechanisms generating and maintaining this important component of biodiversity is to identify plant-herbivore associations. In this study we determined insect-host plant associations for an entire guild of insect herbivores using plant DNA extracted from inse...

Agnimantha plant species have been used in the Ayurvedic system of medicine for many years and is widely as an ingredient ayurvedic formulations. However, source remained controversial it difficult to authenticate from various reports. Hence, present study aims identify its original substitute sources. As per literature sources Clerodendrum phlomidis L.f., C. inerme (L.) Gaertn. Premna serratif...

The majority of the plant materials used in herbal medicine is procured from the markets in the form of dried or powdered plant parts. It is essential to use authentic plant materials to derive the benefits of herbal medicine. However, establishing the identity of these plant materials by conventional taxonomy is extremely difficult. Here we report a case study in which the species identificati...

Abstract DNA barcoding allows the identification of an organism by comparing sequence selected regions (barcodes) with a previously compiled database, and it can be useful for taxonomic species in complex genera, such as Tamarix . Many this genus show convergent morphology, which leads to frequent errors their identification. Highly variable genetic markers, microsatellites or short repeats (SS...

We have studied several samples from Lotus corniculatus s. l. and L. zhegulensis populations with the use of SSR sequenced nuclear (ITS) plastid (atpB-rbcL, ndhC-trnV, rpl32-trnL(UAG), trnH-psbA, trnL-trnF) markers. analysed 9 local Volga River valley ranging Ulyanovsk in north to Volgograd south, as well two Moscow Lipetsk Provinces. Analyses markers using STRUCRURE program divided sample into...

Climate fluctuations in the Quaternary period significantly influenced evolution and distribution of plants, which caused to a serious genetic effects speciation activity [1]. Thus, Central Asia is distinguished by wide variety flora, including 9520 species, while 20% are endemics [2]. Great phenotypic diversity wild flora Uzbekistan, numbering about 4400 species vascular plants determined pecu...

We present a five-locus 129-species phylogeny of Rutaceae from subfamily Zanthoxyloideae, with 193 ingroup samples representing 128 species all 18 genera in the Eriostemon Group and focus on generic infrageneric limits Phebalium its allies. Maximum parsimony Bayesian inference analyses were performed using three plastid markers (psbA–trnH, trnL–trnF, rbcL) two nuclear ribosomal (ITS, ETS). Aste...

With the development of open science and technological innovation, using sharing data molecular biology techniques in study taxonomy systematics have become a crucial component plants, which undoubtedly helps us discover more hidden outliers or deal with difficult taxa. In this paper, we take Dennstaedtia smithii as an example, based on database, virtual herbarium plant photo bank, to clarify t...