Calmodulin has been shown to interact with the COOH-terminal domain of gizzard h-caldesmon at three sites, A (residues 658-666), B (residues 687-695), and B' (residues 717-725), each of which contains a Trp residue [Zhan et al. (1991) J. Biol. Chem. 266, 21810-21814; Marston et al. (1994) J. Biol. Chem. 296, 8134-8139; Mezgueldi et al. (1994) J. Biol. Chem. 269, 12824-12832]. To determine the c...

Deterioration of the environment in which piglets are housed after weaning induces a moderate inflammatory response and modifies tryptophan (Trp) metabolism that can, in turn, decrease Trp availability for growth. We hypothesised that a Trp supply above the current recommendations may be required to preserve Trp availability and to maximise the growth of pigs suffering from moderate inflammatio...

The effects of Ca2+ and substrate analogue binding on the conformational dynamics of porcine pancreas phospholipase A2 (PLA2) in different regions was explored by combining site-directed mutagenesis and time-resolved fluorescence measurements. The single tryptophan residue (Trp-3) of the wild-type protein (W3), in the alpha-helix A, was replaced by a phenylalanine residue (W3F), whereafter Trp ...

Two 4 x 4 Latin square experiments were conducted with English Pointer puppies to determine the growth promoting capacity of D-tryptophan (trp) relative to L-trp and to estimate the trp requirement of the growing dog. Based upon a multiple linear regression slope-ratio technique, D-trp had an efficacy value of 35.5 +/- 6.4% relative to L-trp. The urine of dogs fed D-trp contained 14.6% of the i...

Twenty-five crossbred gilts were used in an experiment to determine the tryptophan (Trp) requirement for reproduction. L-tryptophan was added to a fortified corn-gelatin diet to attain the five Trp levels of .64, 1.04, 1.44, 1.84 and 2.24 g per day. These diets were fed at 2 kg daily during pregnancy I and 1.82 kg daily during pregnancies II and III. During each 3-week lactation, all animals we...

Transient receptor potential (TRP) ion channels play a key role in sensing environmental and endogenous stimuli. Among sensory neurons, different TRP channels are widely expressed, and their expression profiles overlap. Although many TRP channels are homotetramers, we have shown that a functional tetrameric TRP channel can contain two types of monomers.

Store-operated Ca2+ entry, a mode of Ca2+ influx activated by depletion of Ca2+ from the internal stores, has been detected in a wide variety of cell types and may be the primary mechanism for Ca2+ entry in nonexcitable cells. Nevertheless, until recently, no candidate store-operated channel (SOC) had been identified molecularly. Through the serendipity of Drosophila genetics, a candidate SOC, ...

The Drosophila transient receptor potential (trp) gene product (TRP) shows some structural similarity to vertebrate voltage-gated Ca2+ channels. It appears to function as a novel Ca2+ channel responsible for light stimulated, inositol trisphosphate (InsP3)-mediated Ca2+ entry in the fly retina. The subcellular localization of TRP protein was determined in this study using immunohistochemical st...

The kynurenine (KYN) pathway, which is initiated by indoleamine 2,3-dioxygenase (IDO), is a tryptophan (TRP) metabolic pathway. It shares TRP with the serotonin (5-hydroxytryptamine, 5-HT) pathway. In major depression, activation of the KYN pathway may deplete 5-HT. In the present study we investigated the influence of various risk factors for depression, such as ageing, social isolation and ps...

Conventional anti-jamming method mostly rely on frequency hopping to hide or escape from jammer. These approaches are not efficient in terms of bandwidth usage and can also result a high probability jamming. Different existing works, this paper, novel strategy is proposed based the idea deceiving jammer into attacking victim channel while maintaining communications legitimate users safe channel...