Sucrose gradient centrifugation of heat-denatured RNA of turnip yellow mosaic virus permitted the isolation of five RNA classes with molecular weights ranging from 2.0 to 0.25 X 10(6). The infectivity was shown to be confined to an RNA molecule of molecular weight 2.0 X 10(6). No significant increase in infectivity was obtained by combination of the latter RNA with the RNA classes of smaller si...

to evaluate the agronomic characteristics in three brassica species (rape, indian mustard, and turnip) and to determine the best stress tolerance indices, 16 brassica cultivars were studied under controlled conditions in -0.6 mpa of stress and as well under field (either stressed or irrigated) conditions at the dryland agricultural research station, kermanshah during 2004-05. the results showed...

Dye-sensitized solar cells (DSSCs) were assembled by using the bougainvillea flowers, red turnip and the purple wild Sicilian prickly pear fruit juice extracts as natural sensitizers of TiO(2) films. The yellow orange indicaxanthin and the red purple betacyanins are the main components in the cocktail of natural dyes obtained from these natural products. The best overall solar energy conversion...

Turnip crinkle virus is one of several single-stranded RNA plant viruses associated with defective interfering (DI) RNAs. A complete cDNA copy of a 344-base DI RNA (DI RNA G) was cloned downstream from a T7 RNA polymerase promoter. Transcripts synthesized in vitro were infectious when inoculated with helper virus on turnip plants. Studies of the infectivity of DI transcripts containing deletion...

In addition to regulating gene expression, RNA silencing is an essential antiviral defense system in plants. Triggered by double-stranded RNA, silencing results in degradation or translational repression of target transcripts. Viruses are inducers and targets of RNA silencing. To condition susceptibility, most plant viruses encode silencing suppressors that interfere with this process, such as ...

The genomes of positive-strand RNA viruses undergo conformational shifts that complicate efforts to equate structures with function. We have initiated a detailed analysis of secondary and tertiary elements within the 3' end of Turnip crinkle virus (TCV) that are required for viral accumulation in vivo. MPGAfold, a massively parallel genetic algorithm, suggested the presence of five hairpins (H4...

Present investigations were carried out during 1998-2000 at Shivri farm of Uttar Pradesh Bhumi Sudhar Nigam, Lucknow, India, to study the effect of vermicompost application in reclaimed sodic soils on the productivity of potato (Solanum tuberosum), spinach (Spinacia oleracea) and turnip (Brassica campestris). The soil quality was monitored during the experiment followed by productivity. The tre...

The 206-kDa protein of turnip yellow mosaic virus belongs to an expanding group of proteins containing a domain which includes the consensus nucleotide binding site GxxxxGKS/T. A portion of this protein (amino acids [aa] 916 to 1259) was expressed in Escherichia coli and purified by affinity chromatography to near homogeneity. In the absence of any other viral factors, it exhibited ATPase and G...

Using a polyethylene glycol-mediated uptake procedure, protoplasts from a range of Cruciferous species were infected with cauliflower mosaic virus; they include Moricandia arvensis, a plant not k n o w n to be susceptible. With this technique > 90~o of turnip protoplasts were infected, each producing an average of 104 to 105 viral genome equivalents. Filter hybridization and fluorescent antibod...

turnip curly top virus (tctv) is a member of the newly established genus turncurtovirus, family geminiviridae. development of an efficient detection system is necessary to study the biological characteristics of viruses. due to the superior efficacy of the serological methods for detection of plant viruses, in this research, with the aim to facilitate the process of immunological detection of t...