An electrochemical aptasensor for detection of trace amounts of aflatoxin M1 was developed. This required immobilization of aptamer on screen printed gold electrode comprising of working electrode, counter electrode and reference electrode and was achieved by sequentially layering dithiodipropionic acid, streptavidin and biotinylated-tetraethylene glycol-aptamer. Immobilization of aptamer was m...

Kappa carrageenan was identified as a possible gel for melting-triggered release at 30–40 °C with the inclusion of non-ionic surfactant use in detergency or food-related applications. The formulation kappa (0.6–3% w/w) and either Tween 20 Dehypon LS 36: two different surfactants (0–5% w/w). Rheology micro differential scanning calorimetry (µDSC) found that addition had significant increase gell...

Mortierella alpina, a fungus used commercially as a source of arachidonic acid, 20:4(n-6), has been examined to see if growth on lipid-based carbon sources leads to repression of either fatty acid biosynthesis and/or fatty acid desaturation and elongation. Changes in the activities of ATP:citrate lyase, isocitrate lyase, carnitine acetyltransferase, malic enzyme, glucose-6-phosphate dehydrogena...

Measles vaccines were prepared from the same virus fluids by inactivation with formaldehyde or by extraction with ether, ethyl acetate, or Freon 113 in the presence of Tween 80. Tests of antigenic potency, based on antibody levels in guinea pigs, showed that the formaldehyde-inactivated vaccines were more potent than the solvent-inactivated preparations and had the additional advantage of long ...

the aim of this work was to investigate the influence of interactions between modified starch (hi-cap 100) and the nonionic surfactant tween 80 on the properties of cardamom oil-in-water emulsion and its corresponding spray dried microcapsules. to this end the mean diameter and size distribution of droplets as well as their polydispersity index and also the viscosity and flow behavior of emulsi...

The problem of endosulfan bioremediation is poor solubility and restriction of appropriate biocatalyst. One promising approach in increasing the bioavailability of this organic compound is addition of surfactants. The synthetic surfactant Tween 80 was non-toxic to soil microorganisms and inert to the soil matrix and had the additional benefit of causing an enhanced dissolution rate for single c...

Objective Solid dispersions of daidzein (DZ) were prepared using tween-80 as the surfactant to improve its dissolution and solubility. Methods Using tween-80 as the surfactant and polyethylene glycol (PEG)-10000 as the carrier, solid dispersions of DZ were prepared by solvent method. Infrared spectroscopy (IR) and X-ray diffraction spectroscopy were applied to determine the status of DZ in carr...

Sugar parts play important roles in recognizing molecules on the cell membranes. We successfully produced sugar-type micellar surfactants, lactonoalkylamide (LacCn), for the first time. Spherical vesicles, three-component hybrid liposomes, were obtained after the sonication of the mixture of L-alpha-dimyristoyl-phosphatidylcholine (DMPC), Tween 20 and LacCn (DMPC:Tween 20:LacCn=65:7:28). It is ...

Strains of Saccharomyces cerevisiae FL200 capable of growing on a solid medium containing a mixture of polyene macrolide antibiotics (nystatin, 40 micrograms/ml, amphotericin B, 40 micrograms/ml, pimaricin, 150 micrograms/ml and RP9971 antibiotic, 10 micrograms/ml) have been isolated after successive selection steps. The mutant strains, PR13 and PRC24, are 10 to 100 times more resistant than th...

Heretofore most methods for the determination of lipase activity have employed emulsions of fatty acid esters in water (1). These emulsions have been stabilized by use of such agents as gum acacia. The disadvantages of such methods are that (1) the emulsions break up to a considerable and variable extent during the course of the incubation, and (2) the enzyme is presumed to be in the aqueous ph...