N. Leigh Anderson Molecular Anatomy Program Division of Biological and Medical Research Argonne National Laboratory Argonne, Illinois 60439 Received June 6,1979 SUMMARY: Using the technique of thermal denaturation in a temperature gradient followed by two-dimensional gel electrophoresis of the fractions, the thermostabilities of the cytoplasmic actins have been investigated. Addition of 5 mM Mg...

This article concerns the study of stochastic Gilpin-Ayala mutualism models with white noise and Poisson jumps. Firstly, an explicit solution for one-dimensional Gilpin-Ayala mutualism model with jumps is obtained and the asymptotic pathwise behavior is analyzed. Then, sufficient conditions for the existence of global positive solutions, stochastically ultimate boundedness and stochastic perman...

Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study highlights the significance of SDS-TCA procedure over common used methods for the preparation of sa...

PrP(C), the cellular isoform of prion protein, is widely expressed in most tissues. Despite its involvement in several bioprocesses it still has no apparent physiological role. During propagation of Transmissible Spongiform Encephalopathies, PrP(C) is converted to the pathological isoform, PrP(Sc), in a process believed to be mediated by unknown host factors. PrP(Sc) has altered biochemical pro...

In preparing intracellular microbial samples for one- or two-dimensional electrophoresis, trichloroacetic acid (TCA) precipitation is frequently used to remove interfering compounds. Solubilization of TCA precipitate typically requires the addition of a number of chaotropes or detergents, in a multistep process, that requires hours to carry out. In this study, a simple, rapid, one-step method t...

In this paper, an effective numerical method is introduced for the treatment of nonlinear two-dimensional Volterra-Fredholm integro-differential equations. Here, we use the so-called two-dimensional block-pulse functions.First, the two-dimensional block-pulse operational matrix of integration and differentiation has been presented. Then, by using this matrices, the nonlinear two-dimensional Vol...

‎In this paper‎, ‎firstly‎, ‎we review approximation of fuzzy functions‎ ‎by fuzzy bicubic splines interpolation and present a new approach‎ ‎based on the two-dimensional fuzzy splines interpolation and‎ ‎iterative method to approximate the solution of two-dimensional‎ ‎linear fuzzy Fredholm integral equation (2DLFFIE)‎. ‎Also‎, ‎we prove‎ ‎convergence analysis and numerical stability analysis ...