Observations are presented of nonlinear internal waves on the outer New England continental shelf during the summer Shelfbreak Primer study conducted between July 26 and August 5, 1996. Current and temperature measurements were made with an upward looking acoustic Doppler current profiler (ADCP) located on the 147 m isobath near the shelfbreak and three vertical thermistor moorings located upsh...

Name of Sequence. Chicken prolactin (PRL) gene. Genus and Species. Gallus gallus (chicken). Origin of Clones. Genomic DNA from Xing Hua Chinese native chickens was used as a template. Primers were designed based on exon/intron junctions of the genomic turkey PRL gene sequence (Kurima et al., 1995). Polymerase chain reaction (PCR) amplifications of the PRL gene were performed by using the follow...

The Polymerase Chain Reaction (PCR) has facilitated the detection of unculturable microorganisms in virtually any environmental source and has thus been used extensively in the assessment of environmental microbial diversity. This technique relies on the assumption that the gene sequences present in the environment are complementary to the "universal" primers used in their amplification. The re...

Statement of the Problem The clinical success of ceramic depends on the quality of the bond between the zirconia and resin cement. Purpose In the present study, the effects of universal and conventional MDP-containing primers were evaluated on the shear bond strength of zirconia ceramic and nanofilled composite resin. Materials and Method Thirty blocks of zirconia ceramic (6mm×2mm) were pre...

Background: Silane-containing universal adhesives (UAs) are marketed as adhesion promotors for glass-ceramics. Objectives: This study aimed to evaluate the priming capacity of γ-methacryloxypropyltrimethoxysilane (γMPTS)-containing and γ-methacryloxypropyltriethoxysilane (γMPTES)/3-(aminopropyl)triethoxysilane (APTES)-containing lithium disilicate ceramic (LDC). Materials Methods: Etched LDC di...

A method, termed hierarchical oligonucleotide primer extension (HOPE), is developed for quantitative, multiplexing detection of DNA targets present in PCR-amplified community 16S rRNA genes. It involves strand extension reaction and multiple oligonucleotide primers modified with different lengths of polyA at the 5' end and targeting 16S rRNA genes at different phylogenetic specificities. On ann...