the genetic diversity of 89 flue-cured tobacco varieties was examined using 12 issr primers. these cultivarswere evaluated at the guilan tobacco research center, rasht, iran, using a 7×7 simple lattice design with two replications, and 12 morphological traits. the total number of pcr amplified products was 143 bands ranging from 450 to 3000 bp, of which 108 bands (74.28%) were polymorphic. prim...

Seven STMS primers were used to analyse 20 individuals each of 15 populations of North-western Indian Himalayan hull-less barley landraces. A total of 96 alleles were detected of which 40 were common and 56 were rare. Administrative regionwise, highest allelic richness was revealed by populations from Himachal Pradesh (10.71 alleles/locus) followed by Jammu & Kashmir (8.86 alleles/locus) and Ut...

Introduction of graphic representation for nucleotide or protein sequences can provide intuitive overall pictures as well as useful insights for performing large-scale similarity analysis. In this paper, we are analyzing the similarity/dissimilarity of the mitochondrial genome sequences from twenty four mammal species. The analysis is important in finding the relatedness among the species and e...

We have developed a novel algorithm to search for the maximum likelihood tree constructed from amino acid sequences. This algorithm is a variant of genetic algorithms which uses scores derived from the log-likelihood of trees computed by the maximum likelihood method. This algorithm is valuable since it may construct more likely tree from randomly generated trees by utilizing crossover and muta...

Thirty-one species of Mammillaria were selected to study the molecular phylogeny using random amplified polymorphic DNA (RAPD) markers. High amount of mucilage (gelling polysaccharides) present in Mammillaria was a major obstacle in isolating good quality genomic DNA. The CTAB (cetyl trimethyl ammonium bromide) method was modified to obtain good quality genomic DNA. Twenty-two random decamer pr...

In this paper, we introduce a new phylogenetic tree algorithm that generates phylogenetic trees by repeatedly inserting species one-by-one. The incremental phylogenetic tree algorithm can work on proteins or DNA sequences. Computer experiments show that the new algorithm is better than the commonly used UPGMA and Neighbor Joining algorithms. Keywords—Data structure, Distance matrix, Phylogeneti...

In this work, isozyme and agromorphological variability of radio-induced rice mutants with different cytoplasm bases was surveyed. Agromorphological data (plant type, lodging resistance, maturity and yield) were transformed into binary data. These markers, along with isozyme (Peroxidases, Esterases, Catalases, Alcohol Dehydrogenases and Polyphenoloxidases) data, were considered for genetic dive...

?Computer simulations are useful because they can characterize the expected performance of phylogenetic methods under idealized conditions. However, simulation studies are also subject to several sources of bias that make the results of different simulation studies difficult to interpret and often contradictory. In this study, I examined the performance of 26 commonly used methods of phy? logen...

The phylogenetic tree is a widely-used tool to show the evolutionary relationship between taxa. There are many types of trees proposed in literature such as maximum likelihood, neighbor-joining, and UPGMA trees. topologies different not same. Even for same type tree, when they embed nucleotide substitution models, JC69 model, K80 TN93 so on. Although each has its advantages, selecting suitable ...