Most members of the genus Brucella show strong urease activity. However, the role of this enzyme in the pathogenesis of Brucella infections is poorly understood. We isolated several Tn5 insertion mutants deficient in urease activity from Brucella abortus strain 2308. The mutations of most of these mutants mapped to a 5.7-kbp DNA region essential for urease activity. Sequencing of this region, d...

In this study, we have investigated the current situation of Helicobacter pylori infection with a real-time PCR assay, our routine culture method, and a rapid urease test in Southern Denmark. 52 patients who came to gastric endoscopy examination on the two hospitals in Southern Denmark were taken to the study. All the biopsy samples were cultured and further examined for antibiotic resistance i...

Campylobacter pylon or campylobacter like organisms (CLO) are curved bacilli which are found on the gastric mucosa of patients suffering from gastritis, peptic ulceration and other related diseases. These organisms were later renamed as helicobacter pylori. The diagnosis of HP is usually made on histology, culture, urease tests and serology. Although all these tests are 60-100% sensitive and sp...

Soybean (Glycine max [L.] Merrill) mutant aj6 carries a single recessive lesion, aj6, that eliminates ubiquitous urease activity in leaves and callus while retaining normal embryo-specific urease activity. Consistently, aj6/aj6 plants accumulated urea in leaves. In crosses of aj6/aj6 by urease mutants at the Eu1, Eu2, and Eu3 loci, F(1) individuals exhibited wild-type leaf urease activity, and ...

The mechanism of the hypergastrinaemia associated with Helicobacter pylori infection is unknown. It may be an effect of the ammonia produced by the bacterium near the antral epithelial surface. We have examined the effect on serum gastrin of inhibiting H pylori urease activity with acetohydroxamic acid in six duodenal ulcer patients. On day 1 the fasted patients received placebo tablets at 8 am...

The soybean (Glycine max L. [Merrill]) var Itachi has 0.2 to 0.3% the urease activity found in developing embryos of a normal line, Prize. The hydroxyurea sensitivity and pH preference of this basal seed urease indicate that it represents a unique enzyme rather than an unusually low level of the normal seed urease. Itachi's seed urease is less sensitive to hydroxyurea inhibition (65-80% inhibit...

Roots of young soybean (Glycine max [L.] Merr.) plants (up to 25 days old) contain two distinct urease isozymes, which are separable by hydroxyapatite chromatography. These two urease species (URE1 and URE2) differ in: (a) electrophoretic mobility in native gels, (b) pH dependence, and (c) recognition by a monoclonal antibody specific for the seed ("embryo-specific") urease. By these parameters...

during a period of 10 months from may to february 1995, 120 children (72 girls, 48 boys) with an age range of 4-16 years (mean age 10.87, s.d.±2.7) with chronic abdominal pain who had an abnormal endoscopy (gastroduodenal mucosal defect) and positive urease test were treated for h. pylori. patients were treated randomly with either metronidazole and amoxicillin (double therapy, group a) or metr...