Catfish is one of the most important freshwater fish farming commodities in Indonesia. Higher catfish production can be achieved by cultivating transgenic carrying growth hormone (GH) gene African (Clarias gariepinus GH, CgGH). This research focuses on analysis presence CgGH G1, G2, and G3 mutiara broodstock, as an indication stable inheritance. was isolated using RNeasy mini kit RT-PCR. RT-PCR...

The objective of this study was to standardize an HIV Proviral DNA PCR from newly infected HIV-1 individuals. Thirty five consenting, HIV-1 seropositive adults were enrolled in this study. Proviral DNA was extracted by QIAmp® DNA Mini Kit (QIAGEN, Germany) method. Gene testing was performed using Hot Start Polymerase chain reaction (PCR) to avoid non specific amplification. After amplification,...

Developments in forensic genetic profiling mean that only very little DNA is required to generate an identifying profile. However, as this sensitivity increases so does the potential risk of contamination with non-offender DNA, potentially leading to the conviction of innocents, or release of the guilty. Little work has been done on just how much activity is needed to produce this contamination...

4°75′Ε) during May–June 2010. All larvae were euthanized as part of an invasive species eradication project and stored at –20°C until further use. At necropsy, liver tissues were collected, and DNA was extracted by using the Genomic DNA Mini Kit (BIOLINE, London, UK). PCR to detect ranavirus was performed as described by Mao et al. (10). Three samples showed positive results with this PCR. Thes...

Cell-free DNA (cfDNA) in body tissues or fluids is extensively investigated in clinical medicine and other research fields. In this article we provide a direct quantitative real-time PCR (qPCR) as a sensitive tool for the measurement of cfDNA from plasma without previous DNA extraction, which is known to be accompanied by a reduction of DNA yield. The primer sets were designed to amplify a 90 a...

The sea urchin Paracentrotus lividus (Lamarck, 1816) is a keystone herbivore in the Mediterranean Sea due to its ability to transform macroalgal-dominated communities into barren areas characterized by increased cover of bare substrates and encrusting coralline algae, reduced biodiversity and altered ecosystem functions. P. lividus is also an excellent animal model for toxicology, physiology an...

We developed an alternative method to extract DNA and RNA from clotted blood for genomic and molecular investigations. A combination of the TRIzol method and the QIAamp spin column were used to extract RNA from frozen clotted blood. Clotted blood was sonicated and then the QIAamp DNA Blood Mini Kit was used for DNA extraction. Extracted DNA and RNA were adequate for gene expression analysi...

Introducción. La guayaba es uno de los principales frutales la familia Myrtaceae por su alto valor nutricional. Es originario América tropical y países productores son: México, India, Brasil Tailandia. El interés generado en últimos años el mejoramiento genético este cultivo, ha propiciado empleo herramientas moleculares que permitan determinar variabilidad genética seleccionar genes agronómico...

BACKGROUND Confirmatory diagnosis of visceral leishmaniasis (VL), as well as diagnosis of relapses and test of cure, usually requires examination by microscopy of samples collected by invasive means, such as splenic, bone marrow or lymph node aspirates. This causes discomfort to patients, with risks of bleeding and iatrogenic infections, and requires technical expertise. Molecular tests have gr...