BACKGROUND Nonhost resistance is a generalized, durable, broad-spectrum resistance exhibited by plant species to a wide variety of microbial pathogens. Although nonhost resistance is an attractive breeding strategy, the molecular basis of this form of resistance remains unclear for many plant-microbe pathosystems, including interactions with the bacterial pathogen of rice, Xanthomonas oryzae pv...

Hot pepper is one of the economically important crops in Asia. A large number of gene sequences, including expressed sequence tag (EST) and genomic sequences are publicly available. However, it is still a daunting task to determine gene function due to difficulties in genetic modification of a pepper plants. Here, we show the application of the virus-induced gene silencing (VIGS) repression for...

The present study aims at detecting genes expressed at stable levels and contributing to PCD triggered by exogenous oxalic acid (OA) treatment (20 mM, pH 7.0) in tobacco (Nicotiana benthamiana). Qualitative and quantitative time-course analysis of cell death indicated some PCD-like features 24 hrs after OA treatment. Expression of a number of 17 genes contributing to PCD was also examined. Resu...

Ilex dabieshanensis is not only an important ornamental plant, but can also be used to produce Kuding tea, owing its lipid-lowering and anti-inflammatory medicinal properties. The genetic transformation of I. currently difficult, which restricts functional gene studies molecular breeding research on this species. Virus-induced silencing (VIGS) a powerful tool for determining functions in plants...

Trehalose, as a non-reductive disaccharide, plays vital role in plant growth and development resistance to abiotic stress. Trehalose-6-phosphate synthase (TPS) is key enzyme the synthesis mechanism of trehalose TPS1 genes play crucial response stress plants. However, it has rarely been reported that CaTPS1 responds cold salt stresses pepper. To verify function stresses, was silenced by virus-in...

Abstract Background Maize dwarf mosaic virus (MDMV), a member of the genus Potyvirus , infects maize and is non-persistently transmitted by aphids. Several plant viruses have been developed as tools for gene expression silencing in plants. The capacity MDMV both were examined. Results Infectious clones an Ohio isolate MDMV, OH5, obtained, engineered only, simultaneous marker virus-induced (VIGS...

Virus-induced gene silencing (VIGS) can be harnessed to sequence-specifically degrade host transcripts and induce heritable epigenetic modifications referred as virus-induced post-transcriptional (ViPTGS) transcriptional (ViTGS), respectively. Both ViPTGS ViTGS enable manipulation of endogenous expression without the need for transgenesis. Although VIGS has been widely used in many plant specie...