Background: Polycystic ovary syndrome (PCOS) is a multifactorial, heterogeneous, complex genetic endocrine and metabolic disorder, diagnostically characterized by chronic anovulation, polycystic ovaries and biochemical and clinical manifestations of hyperandrogenism. In patients with PCOS lower apoptotic rates found in granulosa cells compared to women with regular ovulatory cycles and this lea...

this study was inducted to elucidate the effect of different steroid hormones on simultaneous maturation of oocytes in caspian salmon. oocytes were incubated with 1, 10, 100 and 1000 ng/ml of testosterone, progesterone and 17 β stradiol, in vitro, for 40 hrs. gvbd was considered as hormones effectiveness. 17β estradiol was effective just in 1000 ng/ml and testosterone had no positive effect on ...

Oocyte maturation and embryo development are controlled by intra-ovarian factors such as steroid hormones. Progesterone (P4) exists in the follicular fluid that contributes to normal mammalian ovarian function and has several critical functions during embryo development and implantation, including endometrial receptivity, embryonic survival during gestation and transformation of the endometrial...

background: therapeutic potential of in vitro maturation (ivm) in infertility is growing with great promise. although significant progress is obtained in recent years, existing ivm protocols are far from favorable results. the first aim of this study was to investigate whether two step ivm manner change reactive oxygen species (ros) and total antioxidant capacity (tac) levels. the second aim wa...

The results of in vitro maturation (IVM) investigations suggest the potential for wider clinical application. This document discusses efficacy IVM as reported published literature to date. replaces same name, last 2013.

background: the effect of temperature and humidity on the maturation of toxocara cati eggs in an in vitro system was investigated. methods: suspensions of toxocara cati eggs, with 5% formalin/saline or 2.5% formalin/ringer were prepared and maintained at 37 °c under 40% humidity or at 25 °c under 98% humidity for 3 weeks for egg development. results: the suspension sample mixed by 2.5% formalin...

background & objectives : different energy substrates and nutrients can greatly influence oocyte nuclear and cytoplasmic maturation. effect of glucose on in-vitro oocyte maturation and subsequent early embryonic development is controversial. the objective of the present study was to verify the effect of various concentrations of glucose on cumulus-oocyte complexes maturation, fertilization and ...

Background: Evaluation of maturation genes expression and their pattern during in vitro culture of vitrified preantral follicles is an essential and could promote either vitrification procedure or culture condition. Materials and Methods: Preantral follicles (with 100- 130 μm diameter) isolated mechanically from 12-14 days old NMRI mice ovaries and divided into vitrification and control groups....

Energy balance is regulated by ghrelin that is a neuroendocrine modulator. Ghrelin is expressed in reproductive organs. However, the role of ghrelin during in vitro maturation and bovine preimplantational development is limited. The purpose of this study was to determine the expression of ghrelin (GHRL) and its receptor growth hormone secretagogue receptor 1A (GHS-R1A) mRNA expression, and cumu...

introduction: dehp [di(2-ethylhexyl) phthalate)] is widely used in plastic industry and some reproductive toxicity has been shown with it. so, this study was designed to evaluate dehp effects on resumption of meiosis and in vitro maturation of mouse oocytes as well as development of embryos resulted from them. material and methods: mice of 4-6 weeks old were administered daily doses of 50, 100,...