this study was conducted to determine polymorphism of gola-drb3 in nadushan goat using pcr-rflp. blood samples were randomly taken from 100 goats. dna was extracted from the blood samples using diatom dna prep kit. spectrophotometer and 1% agarose gel were used for determining its quantities and qualities. exon 2 of drb 3.2 gene encompassing 285 bp was amplified with heminested-pcr method in tw...

this study was carried out to investigate the genetic diversity and population structure of 90 specimens of oxynoemacheilus argyrogramma collected from sepidbarg, gamasiab and ghaleji rivers, in the west of iran. analyses using three microsatellite loci indicated that the average number of alleles in the population was 12, which was well above the reported values for freshwater fishes. the expe...

objective(s): short tandem repeat (str) loci are the most informative dna genetic markers for attempting to individualize biological material for application in paternity and forensic cases. materials and methods: blood samples were collected and the total genomic dna was extracted. the dna samples were used for genotyping vwa and tpox str loci using pcr and polyacrylamide gel electrophoresis. ...

AIMS The purpose of this study was to investigate the correlation between single necleotide polymorphisms (SNPs) of human epidermal growth factor receptor-2 (HER2) gene with osteosarcoma susceptibility in Chinese Han population. METHODS 90 patients with osteosarcoma and 100 healthy controls who were frequency-matched with the former by age and gender were enrolled for a case-control study. 5 ...

AIM To study the contribution of genetic variation in RAD51 to risk of esophageal squamous cell carcinoma (ESCC). METHODS Three single nucleotide polymorphisms (SNPs) in RAD51 (rs1801320, rs4144242 and rs4417527) were genotyped in 316 ESCC patients and 316 healthy controls in Anyang area of China using PCR- RFLP (polymerase chain reaction-restriction fragment length polymorphism). Demographic...

Testing for deviations from Hardy-Weinberg equilibrium is a widely recommended practice for population-based genetic association studies. However, current methods for this test assume a simple random sample and may not be appropriate for sample surveys with complex survey designs. In this paper, the authors present a test for Hardy-Weinberg equilibrium that adjusts for the sample weights and co...

This study aimed to investigate the influence of polymorphisms in chemokine genes, including MCP1, CCR2, and CCR5 with psoriasis vulgaris (PV) risk in a Chinese population.The genotyping of studied polymorphisms through polymerase chain reaction (PCR) and sequencing was conducted in 142 PV patients and 147 healthy controls. The genotype distribution of the polymorphisms in the control group was...

Malaysian arowana (dragonfish; Scleropages formosus) is an ancient osteoglossid fish from southeast Asia. Due to the high demand of the ornamental fish trade and because of habitat loss, the species is close to extinction. We isolated and characterized 10 polymorphic microsatellites of this species, using 5'-anchored PCR. The number of alleles at the 10 microsatellite loci ranged from 2 to 28, ...

Background: Parkinson’s disease (PD) is a complex disorder that involves multiple genetic and environmental factors. Several candidate genes have been speculated to be involved in the development of PD. We conducted a case-control study to investigate the association between PD and single nucleotide polymorphisms (SNP) at the OPRM1, DAT, COMT and DRD2 genes in a Taiwanese population. Methods: T...

OBJECTIVE The interleukin-23 receptor (IL-23R) has been shown to be associated with autoimmune diseases in many different populations. This study aimed to investigate the association between IL-23R gene polymorphism and susceptibility to Hashimoto's thyroiditis (HT) in Chinese Han population of Shandong. METHODS A case-control cohort study was performed in 145 HT patients from First People's ...