This experiment was performed to investigate the effects of exogenous xylanase supplementation on performance, nutrient digestibility and the degradation of non-starch polysaccharides (NSP) in different sections of the gastrointestinal tract (GIT) of broilers fed wheat-based diets. A total of 120 7-day-old Arbor Acres broiler chicks were randomly allotted to two wheat-based experimental diets s...

This study was aimed at enhancing the production of xylanase from an alkaliphilic Bacillus pumilus VLK-1 in submerged fermentation using wheat bran, a cheap and abundantly available agro-residue, through process optimization and to monitor the effect of temperature shift operation on it. The potential of xylanase in saccharification of wheat straw was also investigated. The results showed that ...

To study the working mechanisms for non-starch polysaccharidases to improve the growth performance of broiler chickens, a 21-day feeding trial was conducted. Two dietary treatments were included: 1) wheat diet (the control); 2) wheat+xylanase diet (xylanase, Allzyme PT, Alltech, Kentucky, USA). There were 8 replicates with 8 birds each for each treatment and the experimental diets were given to...

BACKGROUND The filamentous fungus Penicillium oxalicum is a potential alternative to Trichoderma reesei for industrial production of a complete cellulolytic enzyme system for a bio-refinery. Comparative omics approaches can support rational genetic engineering and/or breeding of filamentous fungi with improved cellulase production capacity. In this study, comparative genomic, transcriptomic and...

Two endoxylanases produced by C. acetobutylicum ATCC 824 were purified to homogeneity by column chromatography. Xylanase A, which has a molecular weight of 65,000, hydrolyzed larchwood xylan randomly, yielding xylohexaose, xylopentaose, xylotetraose, xylotriose, and xylobiose as end products. Xylanase B, which has a molecular weight of 29,000, also hydrolyzed xylan randomly, giving xylotriose a...

In the present study Response surface methodology (RSM) was used to investigate the combined effect of relevant process variables to maximize the production of xylanase in submerge fermentation by Penicillium citrinum MTCC 2553. The process variables include pH (6.5, 7.0, and 7.5); temperature (25, 30, and 35°C); agitation speed (190, 200, and 210 rpm); and, substrate (xylan) concentration (0.7...

The present experiment examined the influence of xylanase supplementation and a blend of essential oils (EO; cinnamaldehyde and thymol) on performance and Salmonella horizontal transmission in broiler chickens challenged with Salmonella. Two thousand 1-d-old broiler chicks were randomly assigned to 5 dietary treatments (8 pens/treatment of 50 male broilers each). Four dietary treatments were ch...

An experiment was conducted to study the effects of inclusion of a wheat cultivar (high in nonstarch polysaccharides) and xylanase supplementation of the diet on productive performance, pH of the gastrointestinal tract, nutrient retention, and intestinal enzyme activity of Hy-Line W-36 laying hens from 25 to 47 wk of age. The experiment was completely randomized with 8 treatments arranged facto...

The production of extracellular xylanase by a newly isolated fungus Simplicillium obclavatum MTCC 9604 was studied in solid-state and submerged fermentation. Multiple xylanases and endoglucanases were produced by the strain during growth on wheat bran in solid state fermentation (SSF). A single xylanase isoform was found to be produced by the same fungus under submerged fermentation (SF) using ...

An extracellular xylanase from the thermophilic fungus Melanocarpus albomyces IIS 68 was evaluated for its activity and stability in the presence of polyols and salts at 60 °C, and found to be an effective protecting agent for thermal deactivation of enzyme. Response surface methodology was employed to study the synergistic effects of glycerol and NaCl (thermostabilizers) for xylanase stability...