Differences between the recombinant xylose-utilizing Saccharomyces cerevisiae strain TMB 3399 and the mutant strain TMB 3400, derived from TMB 3399 and displaying improved ability to utilize xylose, were investigated by using genome-wide expression analysis, physiological characterization, and biochemical assays. Samples for analysis were withdrawn from chemostat cultures. The characteristics o...

In recent years, the pentose, d-xylose, has been widely used in the investigation of small bowel function. Most of these tests of xylose absorption depend on the administration of an oral dose of the sugar, followed by an estimation of the xylose levels of the blood or urine. Disease of the upper part of the small bowel has been found to impair xylose absorption; low blood and urine values have...

In the present study, comprehensive, quantitative metabolome analysis was carried out on the recombinant glucose/xylose-cofermenting S. cerevisiae strain MA-R4 during fermentation with different carbon sources, including glucose, xylose, or glucose/xylose mixtures. Capillary electrophoresis time-of-flight mass spectrometry was used to determine the intracellular pools of metabolites from the ce...

BACKGROUND Lignocellulosic biomass is a viable source of renewable energy for bioethanol production. For the efficient conversion of biomass into bioethanol, it is essential that sugars from both the cellulose and hemicellulose fractions of lignocellulose be utilised. RESULTS We describe the development of a recombinant yeast system for the fermentation of cellulose and xylose, the most abund...

Metabolic engineering of Saccharomyces cerevisiae for ethanol production from D-xylose, an abundant sugar in plant biomass hydrolysates, has been pursued vigorously for the past 15 years. Whereas wild-type S. cerevisiae cannot ferment D-xylose, the keto-isomer D-xylulose can be metabolised slowly. Conversion of D-xylose into D-xylulose is therefore crucial in metabolic engineering of xylose fer...

The interaction of D-xylose isomerase purified from two sources with Mn2+ and D-xylose or the competitive inhibitor xylitol has been examined by nuclear magnetic resonance. A greater paramagnetic effect of enzyme-bound Mn2+ on the alpha anomer of D-xylose than on the beta anomer was observed, providing independent evidence for the specificity of D-xylose isomerase for the alpha anomeric form of...

BACKGROUND To engineer Saccharomyces cerevisiae for efficient xylose utilization, a fungal pathway consisting of xylose reductase, xylitol dehydrogenase, and xylulose kinase is often introduced to the host strain. Despite extensive in vitro studies on the xylose pathway, the intracellular metabolism rewiring in response to the heterologous xylose pathway remains largely unknown. In this study, ...

The two metabolically versatile actinobacteria Rhodococcus opacus PD630 and R. jostii RHA1 can efficiently convert diverse organic substrates into neutral lipids mainly consisting of triacylglycerol (TAG), the precursor of energy-rich hydrocarbon. Neither, however, is able to utilize xylose, the important component present in lignocellulosic biomass, as the carbon source for growth and lipid ac...

Escherichia coli contains a cold osmotic shock-releasable binding protein for D-XylOSe which has been purified and characterized. The D-xylose-binding protein has an apparent molecular weight of 37,000 as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and amino acid analysis. D-Xylose-binding protein has an apparent absolute specificity for D-XYlose and binds one molecule o...

effects of excess concentrations of boron on major cell wall components of tobacco cells(nicotiana tabacum l. cv. burley 21) were studied. pectin, xyloglucan, hydroxyproline-richglycoproteins (extensin), and arabinogalactan proteins were characterized. results showedthat increased boron supply resulted in significant decrease in cell and cell wall dry weights.also, high concentrations of boron ...