Comprehensive analysis of protein-protein interactions is a challenging endeavor of functional proteomics and has been best explored in the budding yeast. The yeast protein interactome analysis was achieved first by using the yeast two-hybrid system in a proteome-wide scale and next by large-scale mass spectrometric analysis of affinity-purified protein complexes. While these interaction data h...

Protein recovery under sonication treatment and autolysis, also protein hydrolysis progress during enzymatic hydrolysis (using trypsin and chymotrypsin) and autolysis (using endogenous enzymes) were investigated in Saccharomyces cerevisiae and Kluyveromyces marxianus. Crude protein content of dried yeast cells were 53.22% and 45.6% for S.cerevisiae and K.marxianus, respectively. After 96 hrs of...

zinc (zn) is an essential trace element in all living organisms, and the first eukaryotic zn uptake transporter was discovered in the yeast, saccharomyces cerevisiae. zinc-enriched yeast is a currently available zn supplement. the purpose of the investigation was to compare and evaluate the effect of zn enriched yeast in rainbow trout. the fish (mean body weight 10 ± 0.5 g) were fed a commercia...

plant fatty acyl-acyl carrier protein thioesterase (fat) is a major enzyme regulating the amount and composition of fatty acids in lipids. in this study, one type of cdna, corresponding to the fatty acyl-acyl carrier protein (acyl-acp) thioesterase (fat) enzyme, was isolated from the seed of brassica napus cv. ningyou12. blast results revealed that the cdna identified highly with the fatb class...

The present study aimed to verify whether the inclusion of other ingredients in diet laying hens could interfere with traceability bovine meat and bone meal (BMBM) eggs through stable carbon nitrogen isotope analysis. Two hundred fifty-six white were used randomly distributed into eight treatments composed of: CONTROL: control diet; GLUTEN: + corn gluten; YEAST: yeast; GLUTEN gluten BMBM: 4.5 %...

   In recent science Nanotechnology is a burning field for the researchers. To meet the requirements and growing technological demand, there is a need to develop an eco-friendly approach. In the present effort, the baker’s yeast (Saccharomyces cerevisiae) has been taken in order to assess its potential as putative candidate fungal genera for the transformation of silver nanoparticles. Silve...

HIGHLIGHTS Using date seed powder as Alternative for yeast in rearing of peach fruit fly, Bactrocera zonata. Date provide insects quality parameters corresponding to FAO, IAEA/USDA standards. costs less than yeast.

the hepatitis b surface antigen ( hbsag) gene was isolated from an iranian hbeag positive carrier by pcr. the gene was cloned in puc19 for sequencing and pyes2 for expression in saccharomyces cerevisiae, which pnf1 and pdf3 constructs were made respectively. the sequencing data showed that the isolated hbsag gene shared more than 90% homology with the ayw subtype. the pdf3 was transferred into ...

The topic of microbial interactions is notable relevance in oenology, being connected with their impact on biodiversity and wine quality. among different couples microorganisms, particular yeasts lactic acid bacteria representative the must/wine consortium, have been tested this study. This interaction’s screening has implemented by means plate assays, using culture medium, grape juice, agar as...

Amyloid protein aggregation is a key factor in the development of variety serious diseases humans, commonly named as amyloidoses (Alzheimers and Parkinsons diseases, type II diabetes, etc.), determinant protein-based inheritance lower eukaryotes. In yeast, translation termination Sup35 one most extensively studied amyloidogenic proteins. Aggregation (induction [PSI+] prion) decreases its functi...