We compared the recovery of yeasts from clinical specimens cultured on routine bacteriological media to the recovery of yeast from specimens cultured on a selective fungal medium (Sabouraud agar). The use of Sabouraud agar was especially important in cases of mixed cultures, since in such cases yeast was recovered on bacteriological media from only 50% of 44 yeast-positive pus specimens and fro...

Introduction: Acne is a pathological disorder and a chronic inflammation in the sebaceous follicles, and one of the most popular dermatology damages that has affected millions of people worldwide. The aim of this study was to identify Candida species in patients with acne as well as determine the drug susceptibility of these species.   Materials & Methods: In this cross-sectional study, 70 cl...

This report details the procedural requirements for preparing cell-free extracts of yeast rich in polyribosomes. This enabled us to demonstrate the occurrence of polyribosomes in yeast, to show their role in protein synthesis, and to devise methods for their resolution and isolation. When certain precautions are met (the use of log phase cells, rapidly halting cell growth, gentle methods of dis...

Routine methods for the isolation of Neisseria gonorrhoeae employ various complex media in conjunction with devices designed to supply an atmosphere of approximately 10 per cent carbon dioxide. Recent investigations7 of the CO2 requirement of N. gonorrhoeae have shown that various strains of this microorganism can be grown on a Casamino-salts-glucose agar medium in the absence of CO2, provided ...

Isolation of biologically active cell components from multicellular eukaryotic organisms often poses difficult challenges such as low yields and inability to retain the integrity and functionality of the purified compound. We previously identified a cap-independent translation enhancer (3'CITE) in the 3'UTR of Turnip crinkle virus (TCV) that structurally mimics a tRNA and binds to yeast 80S rib...