Background: Microbiological confirmation of tuberculous meningitis (TBM) remains problematic. We assessed the diagnostic performance of a modified Ziehl-Neelsen (MZN) staining method that showed promise in earlier studies. Methods: Patients evaluated for TBM in Shaanxi province, China, were prospectively enrolled from May, 2011 to April, 2013. Cerebrospinal fluid (CSF) specimens were evaluated ...

Image processing is the most effective method for enhancement and segmentation of tuberculosis bacilli in sputum smear samples. Improper straining can result poor screening results such as over-staining, under-staining, blurred images. The goal to find an image technique that will prepare feature extraction. There are still some shortcomings with existing when it implemented on Ziehl Neelsen In...

The Ziehl–Neelsen (ZN) stain, also known as the acidfast stain, has been reported to be helpful in detection and identification of Schistosoma eggs. In histological sections, Schistosoma mansoni egg shells appear as ZN positive and Schistosoma haematobium shells as ZN negative. The staining target of the responsible ZN component (carbolfuchsin) in the shell is unknown. Because carbolfuchsin is ...

The first description of abacterial urethritis is attributed to Guiard (1897) and his observation was confirmed by Barlow (1899) who also reported that contagious discharges could occur in which no organisms were detectable. Conventionally, when cases of non-gonococcal urethritis are examined by Gram-stained smears, if no organisms are seen the discharge is termed "abacterial"; if a mixture of ...

Cyclospora cayetanensis is a cause of prolonged diarrhoea, mainly in travellers. Laboratory diagnosis may be achieved by a number of methods such as the staining of faecal smears by the modified Ziehl-Neelsen (ZN) technique. Safer methods using this technique have been described for the staining of acid fast bacilli and cryptosporidia by replacing the phenol content of the carbol fuschin stain ...

The patient had a two month history of gastrointestinal symptoms. Upper gastrointestinal endoscopy disclosed 5 mm nodular lesions were seen in the prepyloric area. On pathological examination, two granulomatous lesions were detected in biopsy specimen. Ehrlich Ziehl-Neelsen staining and cultures of the biopsy material were negative, but polymerase chain reaction (PCR) for Mycobacterium tubercul...

The goal of this study was to evaluate the effect of the specimen-processing method that uses the detergent C18-carboxypropylbetaine (CB-18) on the sensitivity of acid-fast bacillus (AFB) staining. Vietnamese immigrants with abnormal chest radiographs provided up to three sputum specimens, which were examined for acid-fast bacilli by use of direct auramine and Ziehl-Neelsen staining. The remain...

AIM To establish the staining characteristics of certain ion exchange resins in histological material, with a view to enabling confident differential identification. METHODS Various histological staining procedures were applied to selected pathological material and prepared agar blocks containing the cation exchange resin calcium polystyrene sulphonate and the anion exchange resin cholestyram...

Fluorescence microscopy for detecting mycobacteria was first used by Hagemann (1) in 1937. Further publications are rare . This method is employed more commonly in smears ( I. 2. 4. 5. K. 12) and rarely in tissue sections (9. 11 ). It gives better results in comparison with the Ziehl-Neel sen stain in both types of material. I n sections of lesions due to leprosy, fluorescence microscopy has no...