Co-injection of zygotes with Cas9 mRNA and sgRNA has been proven to be an efficient gene-editing strategy for genome modification of different species. Genetic engineering in pigs holds a great promise in biomedical research. By co-injection of one-cell stage embryos with Cas9 mRNA and Npc1l1 sgRNA, we achieved precise Npc1l1 targeting in Chinese Bama miniature pigs at the efficiency as high as...

Abstract Most early human embryos contain aneuploid cells. Aneuploidy often arises during the mitotic divisions of embryo, but its origin remains elusive. Human zygotes that cluster their nucleoli at pronuclear interface are thought to be more likely develop into healthy euploid embryos. I will present data showing how parental genomes with in each pronucleus and bovine zygotes. Parental genome...

To evaluate the effect of conditioned media (CM) and Extracellular Vesicles (EVs) derived from bovine oviduct epithelial cell (BOEC) lines on the developmental capacity of bovine zygotes and the quality of embryos produced in vitro, presumptive zygotes were cultured under specific conditions. In experiment 1, zygotes were cultured either on monolayers from BOEC extended culture (E), together wi...

Background: This study was designed to respond to the controversy regarding the necessity of oocyte activation after the ICSI procedure for normal embryo development in sheep. Materials and Methods: The effect of chemical stimulation with either 5mM ionomycin (I) for 5 minutes or ionomycin + 2 mM 6-dimethylaminopurine (6-DMAP) for 3 hours on the efficiency of ICSI, was compared in six experimen...

Abstract Study question Is the behavior of second polar body (PBII) associated with subsequent human embryonic development? Summary answer Human zygotes morphologically static PBII showed significantly higher rates good quality embryos and utilization than showing various behaviors. What is known already In recent years, time-lapse incubators have become widely available, enabling continuous ob...

Gene targeting by homologous recombination in embryonic stem cells is extensively used to generate specific mouse mutants. However, most mammalian species lack tools for targeted gene manipulation. Since double-strand breaks strongly increase the rate of homologous recombination at genomic loci, we explored whether gene targeting can be directly performed in zygotes by the use of zinc-finger nu...

The effect of shifting zygotes from a rich medium into a poor medium (shift-down) on the frequency of recombination has been examined; our results suggest that protein syntheses other than those required for growth may be involved. Experiments with chloramphenicol support this notion, and further suggest that there is a competition between genetic integration and degradation in the recipient ce...

Deletions, duplications, and inversions of large genomic regions covering several genes are an important class of disease causing variants in humans. Modeling these structural variants in mice requires multistep processes in ES cells, which has limited their availability. Mutant mice containing small insertions, deletions, and single nucleotide polymorphisms can be reliably generated using CRIS...