The largest source of mercury located in dental amalgam fillings people. In this study, mercury level in saliva of amalgam and non-amalgam human were compared as well as the level of α amylase has been detected. Materialsand methods: The concentration of mercury, silver, and activity of amylase in saliva were measured in 55 adult study subjects. The mercury contents were determined by cold–vapo...

A thermostable and detergent-stable α-amylase from a newly isolated Anoxybacillus sp. AH1 was purified and characterized. Maximum enzyme production (1874.8 U/mL) was obtained at 24 h of incubation. The amylase was purified by using Sephadex G-75 gel filtration, after which an 18-fold increase in specific activity and a yield of 9% were achieved. The molecular mass of the purified enzyme was est...

Thermostable α-amylases (1,4-α-D-glucan glucanohydrolase; E.C.3.2.1.1) from Bacillus species are of great industrial importance in the production of corn syrup or dextrose. The present study was aimed to determine the effect of temperature, pH, substrate and glucose on stability of α-amylase from Bacillus licheniformis ATCC 6346. To determine the effect of temperature and pH on the stability of...

Priming is one of the most common methods of improving seed quality. The aim of this experiment was to study the effects of hydroand osmopriming on the germination, vigour and hydrolytic enzymes (α-amylase, β-glucosidase, exopeptidase and lipases) activity of zinnia (Zinnia elegans Jacq.) seeds. Seeds of three cultivars: Jowita, Kirke and Orys, and Z. elegans fl.pl., a mixture of cultivars (Mix...

Background: a-Amylases (EC 3.2.1.1) are covering approximately 25% of total enzyme market and are frequently used in food, pharmaceutical and detergent industries. Objectives: The first ever detailed characterization of amylase from any halophilic Engyodontium album is presented. Materials and Methods: An extracellular α-amylase was studied from halophilic E. album TISTR 3645. The enzyme was e...

The antioxidant properties and effect of essential oil of black pepper (Piper guineense) seeds on α -amylase, α -glucosidase (key enzymes linked to type-2 diabetes), and angiotensin-I converting enzyme (ACE) (key enzyme linked to hypertension) were assessed. The essential oil was obtained by hydrodistillation and dried with anhydrous Na2SO4, and the phenolic content, radical [1,1-diphenyl-2 pic...

An obligate halophilic Aspergillus gracilis which was isolated from a hypersaline man-made saltern from Thailand was screened for its potential of producing extracellular α -amylase in the previous studies. In this study the α -amylase was extracted and purified by the help of column chromatography using Sephadex G-100 column. Presence of amylase was verified by SDS-PAGE analysis, showing a sin...

Production of extracellular α-amylase enzyme by a filamentous fungus, Alternaria tenuissima was studied in solid-state fermentation (SSF) as well as submerged fermentation (SmF). The potential strain was successfully mutated by UV and ethyl methanesulfonate (EMS). High-level of α-amylase activity was obtained by the mutant At-Ch-5.6 (76.75 Units mL) after chemical treatment followed by UV mutan...