Nitrogen (N) availability is generally a limiting factor in highly acidic soil, which could be improved by amending these soils with alkaline materials. Soil extracellular enzyme activity (EEA) plays an important role N transformation; current knowledge gap how this occurs amended material. The present 45-day incubation experiment was designed to examine the effects of different amounts materia...

Gaucher disease is a lysosomal storage disorder caused by the deficiency of the lysosomal hydrolase glucocerebrosidase (β-glucocerebrosidase or acid β-D-glucosidase, GBA, EC 3.2.1.45). The deficiency of GBA leads to the accumulation of glucosylceramide (glucocerebroside) in the lysosomes of cells in the monocyte/ macrophage system. In Gaucher disease, glycosphingolipid-engorged cells displace n...

The phytohormone abscisic acid (ABA) plays a critical role in various physiological processes, including adaptation to abiotic stresses. In Arabidopsis thaliana, ABA levels are increased both through de novo biosynthesis and via β-glucosidase homolog1 (BG1)-mediated hydrolysis of Glc-conjugated ABA (ABA-GE). However, it is not known how many different β-glucosidase proteins produce ABA from ABA...

The objective of this work is to investigate the effects of cellulase loading and β-glucosidase supplementation on enzymatic hydrolysis of pretreated Dacotah switchgrass. To assess the difference among various pretreatment methods, the profiles of sugars and intermediates were determined for differently treated substrates. For all pretreatments, 72 h glucan/xylan digestibilities increased sharp...

BACKGROUND The conversion of cellulose by cellulase to fermentable sugars for biomass-based products such as cellulosic biofuels, biobased fine chemicals and medicines is an environment-friendly and sustainable process, making wastes profitable and bringing economic benefits. Trichoderma reesei is the well-known major workhorse for cellulase production in industry, but the low β-glucosidase act...