Mild detergent treatment (0.1% Sarkosyl-0.1% beta-mercaptoethanol) of Dane particle-rich fraction from human serum resulted in the release of core particles together with HBe antigen activity when examined by the reversed passive haemagglutination method. Furthermore, when the core particles isolated by the above procedure were exposed to stronger detergent (1% Sarkosyl-0.1% beta-mercaptoethano...

Disullide bonds in proteins and in seed meals were reduced with /3-mercaptoethanol to sulfhydryl groups. The generated sulfhydryl groups were then alkylated by treatment with 4-vinylpyridine. The cysteine residues were thus derivatized to S-(4-pyridylethyl)-L-cysteine residues. The cysteine derivative is stable to acid hydrolysis and on amino acid analysis by ion exchange elutes just before arg...

We have shown in previous reports that cooked sorghum protein is less digestible than other cooked cereal proteins. The pepsin-indigestible proteins in sorghum were found to be mainly prolamin proteins. Cooking sorghum in the presence of 2-mercaptoethanol increased protein digestibility (in vitro with pepsin or trypsin/chymotrypsin) to a level comparable with other cereals. At a concentration o...

Semisolid culture systems have been developed which permit the proliferation of mousehemopoietic progenitor cells to form colonies of neutrophils and macrophages (1, 2), erythropoietic cells (3), eosinophils (4), and megakaryocytes (5) . These cloning systems have made it possible to analyse in detail many aspects of the nature and control of these hemopoietic populations . With a cloning syste...

CLINICAL CHEMISTRY, Vol. 23, No. 2, 1977 305 captoethanol was used per liter. We consider that this is due to the known tendency of mercaptoethanol to form aggregates with immunoglobulins, mainly IgG3 and 1gM (3-5). This observation was confirmed when we investigated the effect of two concentrations of mercaptoethanol (2.5 X i0 and 4.0 X 10 mol/liter) on the serum proteins, including immunoglob...

It is now commonly accepted that thiol activated creatine kinase (CK) assay systems measure CK activity more accurately and more reproducibly than non-activated systems. However, some differences have arisen in the literature in regards to the nature and course of CK activation. Some thiol compounds have been reported to yield higher enzyme activities than others. Dalal et al. (1) reported that...

The lipase producing Aspergillus sp. ST11 was identified by molecular and morphological methods. primers ITS1/ITS4 were used for amplifying the ITS region. It showed that strain grouped with oryzae flavus (98 % bootstrap value). colony morphology of on malt extract agar Czapek yeast a characteristic A. oryzae. Therefore, it as ST11. produced purified characterized. purification steps involved p...

The opportunistic fungal pathogens belonging to the Candida haemulonii complex and phylogenetically related species auris are well-known for causing infections that difficult treat due their multidrug-resistance profiles. is even more worrisome its ability cause outbreaks in healthcare settings. These emerging yeasts produce a wide range of virulence factors facilitate development infectious pr...