نتایج جستجو برای: آغازگر ssr

تعداد نتایج: 8452  

2016
Jin-Hee Kim Jun-Hoi Kim Won-Sam Jo Jeong-Gwan Ham Il Kyung Chung Kyung-Min Kim

In this study, a cDNA library was constructed from the total RNA of sweet potato leaves. A total of 789 copies of the cDNA were cloned in Escherichia coli by employing the pGEM-T Easy vector. Sequencing was carried out by Solgent Co. (Korea). As many as 579 expressed sequence tag-simple sequence repeat (EST-SSR) markers were designed (73.38%) from the known cDNA nucleotide base sequences. The l...

Journal: :Neurology India 2006
Barin Selçuk Murat Ersoz Murat Inanir Aydan Kurtaran Müfit Akyuz

BACKGROUND AND AIMS To investigate whether there were changes in the sympathetic skin responses (SSR) in the limbs with complex regional pain syndrome (CRPS) type I in hemiplegic patients. SETTING A physical medicine and rehabilitation center in Turkey. MATERIALS AND METHODS Sympathetic skin responses were evaluated in 69 stroke patients (41 with CRPS and 28 without CRPS) and 20 healthy vol...

Journal: :Iranian journal of medical sciences 2016
Mohamadreza Emad Sharareh Roshanzamir Alireza Dabbaghmanesh Mohsen Zafar Ghasempoor Heidar Eivazlou

It is more than a decade since scientists are making use of sympathetic skin response (SSR) as a clinical and research method to evaluate sympathetic nervous system. A major portion of the efferent pathway of this response is composed of non-myelinated nerves. Thus, the latency of the response may be significantly different in normal individuals with different height and limb lengths. This stud...

2005
M. Cala

The factor of safety for slopes (FS) has been traditionally evaluated using two-dimensional limit equilibrium methods (LEM). However the FS of a slope can also be computed with FLAC by reducing the soil shear strength in stages until the slope fails. This method is called the shear strength reduction technique (SSR). Many authors have pointed out several advantages of SSR over the limit equilib...

2012
Stéphanie Barthe Felix Gugerli Noelle A. Barkley Laurent Maggia Céline Cardi Ivan Scotti

Simple sequence repeat (SSR) markers are widely used tools for inferences about genetic diversity, phylogeography and spatial genetic structure. Their applications assume that variation among alleles is essentially caused by an expansion or contraction of the number of repeats and that, accessorily, mutations in the target sequences follow the stepwise mutation model (SMM). Generally speaking, ...

Journal: :The Journal of Cell Biology 1990
D Görlich S Prehn E Hartmann J Herz A Otto R Kraft M Wiedmann S Knespel B Dobberstein T A Rapoport

Bifunctional cross-linking reagents were used to probe the protein environment in the ER membrane of the signal sequence receptor (SSR), a 24-kD integral membrane glycoprotein (Wiedmann, M., T. V. Kurzchalia, E. Hartmann, and T. A. Rapoport. 1987. Nature [Lond.]. 328:830-833). The proximity of several polypeptides was demonstrated. A 22-kD glycoprotein was identified tightly bound to the 34-kD ...

Journal: :Genetics and molecular research : GMR 2015
X Lu H Zhou Y-B Pan C Y Chen J R Zhu P H Chen Y-R Li Q Cai R K Chen

No information is available on segregation analysis of DNA markers involving both pollen and self-progeny. Therefore, we used capillary electrophoresis- and fluorescence-based DNA fingerprinting together with single pollen collection and polymerase chain reaction (PCR) to investigate simple sequence repeat (SSR) marker segregation among 964 single pollens and 288 self-progenies (S1) of sugarcan...

ژورنال: :دوفصلنامه فن آوری زیستی در کشاورزی(علمی-پژوهشی) 2014
دینا کبریایی محمدحسین رضادوست مجتبی کردرستمی حبیب الله سمیع زاده لاهیجی

به­منظور مطالعه تنوع ژنتیکی ژرم پلاسم، 74 ژنوتیپ برنج انتخاب شده و با استفاده از 56 جفت آغازگر ssr مورد تجزیه قرار گرفتند. تمامی آغازگرها مناطقی از ژنوم برنج را تکثیر نمودند که در مجموع 399 آلل چندشکل با میانگین 13/7 آلل به ازای هر جایگاه ریزماهواره در ژنوتیپ ها تکثیر شد. نشانگرهای rm16، rm3355 و rm8006 با 10 آلل بیشترین تعداد آلل را دارا بودند. میزان اطلاعات چندشکلی برای نشانگرهای مورد بررسی ا...

2015
Rusama Marubodee Eri Ogiso-Tanaka Takehisa Isemura Sompong Chankaew Akito Kaga Ken Naito Hiroshi Ehara Norihiko Tomooka Xianlong Zhang

Vigna vexillata (L.) A. Rich. (tuber cowpea) is an underutilized crop for consuming its tuber and mature seeds. Wild form of V. vexillata is a pan-tropical perennial herbaceous plant which has been used by local people as a food. Wild V. vexillata has also been considered as useful gene(s) source for V. unguiculata (cowpea), since it was reported to have various resistance gene(s) for insects a...

سیب شرقی (Malusorientalis) دارای پراکنش وسیع در سرتاسر جنگل­های هیرکانی شمال ایران از مناطق پایین‌بند تا نواحی کوهستانی و شیب‌دار است. برای بررسی تنوع ژنتیکی، نمونه‌های برگ 60 پایه از شش رویشگاه جنگل‌های هیرکانی جمع‌آوری شدند. DNAهای استخراج شده با استفاده از شش آغازگر GD142، GD12، GD147 GD96، GD100 و GD162 به­روش SSR-PCR بررسی شدند. کل آلل­های تکثیر­شده برای چهار آغازگر در ای...

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